Archives of Virology

, Volume 159, Issue 6, pp 1431–1438 | Cite as

Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China

  • Shanyi Chen
  • Ying Zhou
  • Ting Ye
  • Lu Hao
  • Liyun Guo
  • Zaifeng Fan
  • Shifang Li
  • Tao Zhou
Brief Report


In this study, we generated sequences of the apple chlorotic leaf spot virus (ACLSV) coat protein (CP) gene. Genetic variation and phylogenetic analyses were carried out on these sequences along with others reported previously. ACLSV populations clustered into four types: in three of the four types, combinations of three amino acids at positions of 40, 75 and 79 were conserved. The fourth phylogenetic type, newly identified here, was characterized by co-variation of Ser40-Tyr75-Ser79. Statistically significant genetic differentiation and infrequent gene flow were detected among the four types. Two natural recombinants were detected for the first time among ACLSV isolates/genotypes from China.


Trichovirus Molecular variance Recombinant Pathogenicity 



The authors thank the local technicians in the principal apple-producing regions in China for assistance with sample collection. This research was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (nycytx-08-04-02), the Special Fund for Agro-Scientific Research in the Public Interest (No. 201203076-02) administered by the Chinese Ministry of Agriculture, the National High Technology Research and Development Program (863) of China (2012AA101501), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1042).

Supplementary material

705_2013_1927_MOESM1_ESM.doc (2.8 mb)
Supplementary material 1 (DOC 2823 kb)


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Shanyi Chen
    • 1
  • Ying Zhou
    • 1
  • Ting Ye
    • 1
  • Lu Hao
    • 1
  • Liyun Guo
    • 1
  • Zaifeng Fan
    • 1
  • Shifang Li
    • 2
  • Tao Zhou
    • 1
  1. 1.State Key Laboratory of Agrobiotechnology and Department of Plant PathologyChina Agricultural UniversityBeijingChina
  2. 2.State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina

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