Structural requirements of glycosaminoglycans for their interaction with HIV-1 envelope glycoprotein gp120
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Heparan sulfate proteoglycans are known to assist HIV-1 entry into host cells, mediated by the viral envelope glycoprotein gp120. We aimed to determine the general structural features of glycosaminoglycans that enable their binding to gp120, by surface plasmon resonance. Binding was found to be dependent on sequence type, size and sulfation patterns. HIV-1 gp120 prefers heparin and heparan sulfate (with at least 16 monomers in length) over chondroitin and dermatan. Sulfate groups were essential to promote this interaction. These results advance the understanding of the molecular-level requirements for virus attachment and cell entry.
KeywordsHIV-1 gp120 Viral attachment Heparan sulfate proteoglycans Glycosaminoglycans Surface plasmon resonance
This work was funded by Fundação para a Ciência e a Tecnologia–Ministério da Educação e Ciência (FCT-MEC, Portugal; projects PTDC/QUI-BIQ/104787/2008 and VIH/SAU/0047/2011, as well as P.M.M. PhD fellowship SFRH/BD/42205/2007) and the Spanish Ministry of Science and Innovation (project BIO2009-08983 to R.G.G.).
- 5.Esko JD, Kimata K, Lindahl U (2009) Proteoglycans and Sulfated Glycosaminoglycans. In: Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, Hart GW, Etzler ME (eds) Essentials of Glycobiology. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- 7.Roderiquez G, Oravecz T, Yanagishita M, Bou-Habib DC, Mostowski H, Norcross MA (1995) Mediation of human immunodeficiency virus type 1 binding by interaction of cell surface heparan sulfate proteoglycans with the V3 region of envelope gp120-gp41. J Virol 69:2233–2239PubMedCentralPubMedGoogle Scholar
- 17.Rider CC, Coombe DR, Harrop HA, Hounsell EF, Bauer C, Feeney J, Mulloy B, Mahmood N, Hay A, Parish CR (1994) Anti-HIV-1 activity of chemically modified heparins: correlation between binding to the V3 loop of gp120 and inhibition of cellular HIV-1 infection in vitro. Biochemistry 33:6974–6980PubMedCrossRefGoogle Scholar