Archives of Virology

, Volume 159, Issue 2, pp 267–275 | Cite as

Combined administration of oseltamivir and hochu-ekki-to (TJ-41) dramatically decreases the viral load in lungs of senescence-accelerated mice during influenza virus infection

  • Eriko Ohgitani
  • Masakazu Kita
  • Osam Mazda
  • Jiro Imanishi
Original Article


To enhance the effect of anti-influenza-virus agent treatment, the effect of combined administration of oseltamivir phosphate and hochu-ekki-to (Japanese traditional herbal medicine, HET) on early viral clearance was examined. Senescence-accelerated mice were given HET in drinking water for 2 weeks, followed by intranasal infection with influenza A virus strain PR8. After 4 hours of infection, oseltamivir was administered orally for 5 days. The viral loads in the lungs of the group receiving combined treatment were dramatically lower when compared with the viral loads in the lungs of the group receiving oseltamivir alone. HET significantly increased the induction of IL-1β and TNF-α in the lungs of PR8-infected mice and stimulated alveolar macrophage phagocytosis. From these results, we conclude that these functions may be responsible the increased effect on viral load reduction. Here, we show that the combined administration of oseltamivir and HET is very useful for influenza treatment in senescence-accelerated mice.



This work was supported by JSPS KAKENHI Grant Number 19590697 and Japan Kampo Medicines Manufacturers Association.

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Eriko Ohgitani
    • 1
  • Masakazu Kita
    • 1
  • Osam Mazda
    • 1
  • Jiro Imanishi
    • 2
  1. 1.Department of Immunology, Graduate School of MedicineKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Meiji University of Integrative MedicineKyotoJapan

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