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Archives of Virology

, Volume 158, Issue 12, pp 2523–2530 | Cite as

Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus

  • Qinghua Wang
  • Liangjian Qu
  • Zhilin Zhang
  • Yuzhu Wang
  • Yongan Zhang
Original Article

Abstract

Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DkNPV) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against D. kikuchii. In this work, a 1755-bp DkChi gene with sequence homology to a chitinase gene was cloned from the genomic DNA of DkNPV using a DNA fragment library. The DkChi gene, encoding 558 residues protein with a predicted mass of 61.6 kDa, was expressed at high levels in Escherichia coli and purified by affinity chromatography. We confirmed that the prepared protein was the DkChi protein by mass spectrometry analysis. Enzyme activity analysis showed that DkChi had both endo- and exo-chitinase activities. Interestingly, the DkChi protein displayed a strong insecticidal activity against Spodoptera exigua, Hyphantria cunea, Helicoverpa armigera and Lymantria dispar. The results suggest that DkChi is a good candidate protein for significantly contributing to pest control.

Keywords

Chitin Chitinases GlcNAc Insecticidal Activity Peritrophic Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the Program of National Nature Science Foundation of China (grant no. 31200491) and State 863 Project funded by Ministry of Science and Technology (grant no. 2012AA101503), P.R. China.

Supplementary material

705_2013_1775_MOESM1_ESM.tif (292 kb)
Fig. S1 Phylogenetic tree of chitinases from baculovirus. The tree was constructed by the neighbor-joining method, and bootstrap values are indicated at the branches. The amino acid sequences of chitinases were from the following viruses: Dendrolimus kikuchii NPV (JN680874), Hyphantria cunea NPV (YP473218), Orgyia pseudotsugata MNPV (NP046280), Bombyx mori NPV (NP047523), Autographa californica MNPV (NP054156), Spodoptera exigua MNPV (NP037779), Helicoverpa armigera SNPV (AAK28347) and Spodoptera litura NPV (NP258310) (TIFF 292 kb)

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Qinghua Wang
    • 1
  • Liangjian Qu
    • 1
  • Zhilin Zhang
    • 2
  • Yuzhu Wang
    • 1
  • Yongan Zhang
    • 1
  1. 1.The Key Laboratory of Forest Protection, State Forestry Administration of China, Research Institute of Forest Ecology, Environment and ProtectionChinese Academy of ForestryBeijingPeople’s Republic of China
  2. 2.Wulanchabu Forest Disease and Pest Control and Quarantine StationWulanchabuPeople’s Republic of China

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