Characterization of a new apscaviroid from American persimmon
- 449 Downloads
A unique circular molecule of 358 nucleotides was detected in American persimmon (Diospyros virginiana L.). The molecule was graft-transmissible and had genetic characteristics of members of the genus Apscaviroid. It had the highest sequence similarity (72-73 %) to citrus viroid VI (CVd-VI) and formed a clade with CVd-VI, citrus dwarfing viroid, and apple dimple fruit viroid in a phylogenetic tree. The molecule was not detected in citrus, unlike CVd-VI, which infects citrus and persimmon, and it was genetically distant from persimmon latent viroid, which infects persimmon only. The genetic and biological features indicated that the molecule may be a member of a new apscaviroid species.
KeywordsCircular Molecule Deep Sequencing Analysis Japanese Persimmon Decline Symptom Species Demarcation Criterion
The authors thank Dr. A. Kono for persimmons, and Dr. Nakaune for technical advice.
- 5.Keese P, Visvader JE, Symon RH (1988) Sequence variability in plant viroid RNAs. In: Domingo E, Holland JJ, Ahlquist P (eds) RNA genetics volume III. CRC Press, FL, pp 71–98Google Scholar
- 8.Owens RA, Flores R, Di Serio F, Li S-F, Pallás V, Randles JW, Sano T, Vidalakis G (2012) Viroids. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the international committee on taxonomy of viruses. Academic Press, San Diego, CA, pp 1221–1234Google Scholar