Archives of Virology

, Volume 158, Issue 12, pp 2629–2631 | Cite as

Characterization of a new apscaviroid from American persimmon

  • Takao Ito
  • Koichi Suzaki
  • Masaaki Nakano
  • Akihiko Sato
Annotated Sequence Record


A unique circular molecule of 358 nucleotides was detected in American persimmon (Diospyros virginiana L.). The molecule was graft-transmissible and had genetic characteristics of members of the genus Apscaviroid. It had the highest sequence similarity (72-73 %) to citrus viroid VI (CVd-VI) and formed a clade with CVd-VI, citrus dwarfing viroid, and apple dimple fruit viroid in a phylogenetic tree. The molecule was not detected in citrus, unlike CVd-VI, which infects citrus and persimmon, and it was genetically distant from persimmon latent viroid, which infects persimmon only. The genetic and biological features indicated that the molecule may be a member of a new apscaviroid species.


Circular Molecule Deep Sequencing Analysis Japanese Persimmon Decline Symptom Species Demarcation Criterion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Dr. A. Kono for persimmons, and Dr. Nakaune for technical advice.


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Takao Ito
    • 1
  • Koichi Suzaki
    • 1
  • Masaaki Nakano
    • 2
  • Akihiko Sato
    • 1
  1. 1.Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS)National Agriculture and Food Research Organization (NARO)HiroshimaJapan
  2. 2.Okitsu Citrus Research StationNIFTS, NAROShizuokaJapan

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