Archives of Virology

, Volume 158, Issue 10, pp 2175–2177 | Cite as

Culicoides sonorensis (Diptera: Ceratopogonidae) is not a competent vector of Cache Valley virus (family Bunyaviridae, genus Orthobunyavirus)

Brief Report


We investigated the susceptibility of Culicoides sonorensis to Cache Valley virus (CVV) (family Bunyaviridae, genus Orthobunyavirus) infection and the potential that it could be a vector or site of virus reassortment. CVV is native to the New World and causes disease in livestock. Infected blood meals were fed to both a competent vector, Anophelesquadrimaculatus, and Culicoides sonorensis. All Anopheles mosquitoes were infected as expected, but only 21 % of the C. sonorensis insects were susceptible to infection. These appeared to present a midgut barrier, because virus persisted but did not disseminate. This means Culicoides sonorensis is not likely to be a vector of CVV but could be involved in viral reassortment. Schmallenberg virus (SBV) (family Bunyaviridae, genus Orthobunyavirus) was recently discovered in Europe and probably is a novel virus resulting from a reassortment of two orthobunyaviruses, and an ongoing epizootic in cattle and small ruminants has caused significant economic damage.


Schmallenberg virus Cache Valley virus Culicoides sonorensis Vector competence 


  1. 1.
    Edwards JF, Livingston CW Jr, Chung SI, Collisson EC (1989) Ovine arthrogryposis and central nervous system malformations associated with in utero Cache Valley virus infection: spontaneous disease. Vet Pathol 26:33–39PubMedCrossRefGoogle Scholar
  2. 2.
    Chung SI, Livingston CW Jr, Edwards JF, Gauer BB, Collisson EC (1990) Congenital malformations in sheep resulting from in utero inoculation of Cache Valley virus. Am J Vet Res 51:1645–1648PubMedGoogle Scholar
  3. 3.
    Neitzel DF, Grimstad PP (1991) Serological evidence of California group and Cache Valley virus infection in Minnesota white-tailed deer. J Wild Dis 27:230–237Google Scholar
  4. 4.
    Sahu SP, Pedersen DD, Ridpath HD, Ostlund EN, Schmitt BJ, Alstad DA (2002) Serologic survey of cattle in the northeastern and north central United States, Virginia, Alaska, and Hawaii for antibodies to Cache Valley and antigenically related viruses (Bunyamwera serogroup virus). Am J Trop Med Hyg 67:119–122PubMedGoogle Scholar
  5. 5.
    Campbell GL, Mataczynski JD, Reisdorf ES, Powell JW, Martin DA, Lambert AJ, Haupt TL, Davis JP, Lanciotti RS (2006) Second human case of Cache Valley virus disease. Emerg Inf Dis 12:854–856CrossRefGoogle Scholar
  6. 6.
    Holden P, Hess AD (1959) Cache Valley Virus, a previously undescribed mosquito-borne agent. Sci 130:1187–1188CrossRefGoogle Scholar
  7. 7.
    Calisher CH, Sabattini MS, Monath T, Wolff K (1988) Cross-neutralization tests among Cache Valley virus isolates revealing the existence of multiple serotypes. Am J Trop Med Hyg 39:202–205PubMedGoogle Scholar
  8. 8.
    Blackmore CGM, Blackmore MS, Grimstad PR (1998) Role of Anopheles quadrimaculatus and Coquillettida perturbans (Diptera: Culicidae) in the Transmission Cycle of Cache Valley Virus (Bunyaviridae: Bunyavirus) in the Midwest, U.S.A. J Med Entomol 35:660–664PubMedGoogle Scholar
  9. 9.
    Garigliany M, Bayrou C, Kleijnen D, Cassart D, Jolly S, Linden A, Desmecht D (2012) Schmallenberg virus: A new Shamonda/Sathuperi-like virus on the rise in Europe. Antiviral Res.
  10. 10.
    Peperkamp K, Dijkman R, van Maanen C, Vos J, Wouda W, Holzhauer M, van Wuijckhuise L, Junker K, Greijdanus S, Roumen M (2012) Polioencephalo- myelitis in a calf due to infection with Schmallenberg virus. Vet Rec 170:570PubMedCrossRefGoogle Scholar
  11. 11.
    Yanase T, Kato T, Aizawa M, Shuto Y, Shirafuji H, Yamakawa M, Tsuda T (2012) Genetic reassortment between Sathuperi and Shamonda viruses of the genus Orthobunyavirus in nature: implications for their genetic relationship to Schmallenberg virus. Arch Virol.
  12. 12.
    Rasmussen LD, Kristensen B, Kirkeby C, Rasmussen TB, Belsham GJ, Bodker R, Botner A (2012) Culicoids as vectors of Schmallenberg virus. Emerg Infect Dis 18:1204–1206PubMedCrossRefGoogle Scholar
  13. 13.
    Borkent A (2005) The biting midges, the Ceratopogonidae (Diptera).Pp 113126. In: Marquardt WC (ed) Biology of Disease Vectors, 2nd edn. Elsevier Press, AmsterdamGoogle Scholar
  14. 14.
    Hunt GJ. (1994) A procedural manual for the large-scale rearing of the biting midge, Culicoides variipennis (Diptera: Ceratopogonidae).In: US Department of Agriculture, Agricultural Research Service, ARS-121, pp 1–68. National Technical Information Service, SpringfieldGoogle Scholar
  15. 15.
    Moriarity JR, Loftis AD, Dasch GA (2005) High-throughput molecular testing of ticks using a liquid-handling robot. J Med Entomol 42:1063–1067PubMedCrossRefGoogle Scholar
  16. 16.
    Bowen MD, Trappier SG, Sanchez AJ, Meyer RF, Goldsmith CS, Zak SR, Dunster LM, Peters CJ, Ksiazek TG, Nichol ST, Task Force RVF (2001) A reassortant Bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia. Virol 291:185–190CrossRefGoogle Scholar
  17. 17.
    Service MW (1968) Blood digestion and oviposition in Culicoides impunctatus Goetghebuer and C. obsoletus (Meigen) (Diptera, Ceratopogonidae). Ann Trop Med Parasitol 62:325–330PubMedGoogle Scholar
  18. 18.
    Hussein AE, Ramig RF, Holbrook FR, Beaty BJ (1989) Asynchronous mixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17. J Gen Virol 70:3355–3362PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien (outside the USA) 2013

Authors and Affiliations

  1. 1.US Air Force School of Aerospace Medicine PHRWright-Patterson AFBUSA
  2. 2.Wyoming State Veterinary LaboratoryUniversity of WyomingLaramieUSA

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