Archives of Virology

, Volume 158, Issue 10, pp 2157–2162

A divergent clade of circular single-stranded DNA viruses from pig feces

  • Andrew K. Cheung
  • Terry F. Ng
  • Kelly M. Lager
  • Darrell O. Bayles
  • David P. Alt
  • Eric L. Delwart
  • Roman M. Pogranichniy
  • Marcus E. KehrliJr.
Brief Report

DOI: 10.1007/s00705-013-1701-z

Cite this article as:
Cheung, A.K., Ng, T.F., Lager, K.M. et al. Arch Virol (2013) 158: 2157. doi:10.1007/s00705-013-1701-z

Abstract

Using metagenomics and molecular cloning methods, we characterized five novel small, circular viral genomes from pig feces that are distantly related to chimpanzee and porcine stool-associated circular viruses, (ChiSCV and PoSCV1). Phylogenetic analysis placed these viruses into a highly divergent clade of this rapidly growing new viral family. This new clade of viruses, provisionally named porcine stool-associated circular virus 2 and 3 (PoSCV2 and PoSCV3), encodes a stem–loop structure (presumably the origin of DNA replication) in the small intergenic region and a replication initiator protein commonly found in other biological systems that replicate their genomes via the rolling–circle mechanism. Furthermore, these viruses also exhibit three additional overlapping open reading frames in the large intergenic region between the capsid and replication initiator protein genes.

Keywords

Deep sequencing Porcine stool-associated circular virus 

Copyright information

© Springer-Verlag Wien (outside the USA) 2013

Authors and Affiliations

  • Andrew K. Cheung
    • 1
  • Terry F. Ng
    • 2
  • Kelly M. Lager
    • 1
  • Darrell O. Bayles
    • 3
  • David P. Alt
    • 3
  • Eric L. Delwart
    • 2
  • Roman M. Pogranichniy
    • 4
  • Marcus E. KehrliJr.
    • 1
  1. 1.Virus and Prion Diseases, Research UnitNational Animal Disease Center, USDA, Agricultural Research ServiceAmesUSA
  2. 2.Department of Laboratory Medicine, Blood Systems Research InstituteUniversity of California at San FranciscoSan FranciscoUSA
  3. 3.Infectious Bacterial Diseases, Research UnitNational Animal Disease Center, USDA, Agricultural Research ServiceAmesUSA
  4. 4.Indiana Animal Disease Diagnostic Laboratory and Department of Comparative PathobiologyPurdue UniversityWest LafayetteIndiana

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