Archives of Virology

, Volume 158, Issue 9, pp 1943–1952 | Cite as

Molecular characterization of five betacryptoviruses infecting four clover species and dill

  • Till Lesker
  • Frank Rabenstein
  • Edgar Maiss
Original Article


The family Partitiviridae includes plant (Alphacryptovirus and Betacryptovirus), fungal (Partitivirus) and protozoan (Cryspovirus) viruses with bisegmented dsRNA genomes and isometric virions. Cryptic viruses commonly occur in different plant species without causing any symptoms. So far, numerous sequences have been determined for viruses of the genus Alphacryptovirus, but no sequence is available for any assigned member of the genus Betacryptovirus. Following extraction, cloning and sequence analysis of double-stranded RNA in this study, we report the molecular properties of three assigned members of the genus Betacryptovirus, white clover cryptic virus 2, red clover cryptic virus 2 and hop trefoil cryptic virus 2, and two new putative betacryptoviruses found in crimson clover and dill. Betacryptoviruses share sequence motifs with members of the genus Partitivirus. In phylogenetic analyses, members of the genus Betacryptovirus formed a new sub-cluster within the clusters containing members of the genus Partitivirus. Our results provide evidence for a distinct evolutionary lineage of dsRNA viruses of plants and fungi.


Coat Protein White Clover Fungal Virus dsRNA Segment Crimson Clover 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Dr. Piero Caciagli from the Istituto di Virologia Vegetale, Torino, Italia, for providing the WCCV-2 antiserum.


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  1. 1.Institute of Plant Diseases and Plant ProtectionLeibniz Universität HannoverHannoverGermany
  2. 2.Julius Kühn Institute, Federal Research Centre for Cultivated Plants (JKI)Institute of Epidemiology and Pathogen DiagnosticsQuedlinburgGermany

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