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Archives of Virology

, Volume 158, Issue 8, pp 1829–1832 | Cite as

Molecular characterization of a new alphasatellite associated with a cassava mosaic geminivirus in Madagascar

  • Mireille Harimalala
  • Alexandre De Bruyn
  • Murielle Hoareau
  • Alice Andrianjaka
  • Sahondramalala Ranomenjanahary
  • Bernard Reynaud
  • Pierre Lefeuvre
  • Jean-Michel LettEmail author
Annotated Sequence Record

Abstract

Two complete nucleotide sequences of an alphasatellite isolated from a cassava plant with mosaic disease symptoms in Madagascar are described and analyzed. While the helper begomovirus was identified as an isolate of East African cassava mosaic Kenya virus (EACMKV), its associated alphasatellite was most closely related (80 % nucleotide sequence identity) to cotton leaf curl Gezira alphasatellite. These satellite molecules have typical features of alphasatellites, with a single gene in the virion sense, an A-rich region and a stem-loop structure. According to the proposed species demarcation threshold of alphasatellites (83 % nucleotide identity), they are isolates of a new species for which we propose the name “Cassava mosaic alphasatellite”.

Keywords

Cassava Plant Cassava Mosaic Disease African Cassava Mosaic Virus Virion Sense Satellite Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was funded by the European Union (FEDER), the Région Réunion and CIRAD. MH is a recipient of a PhD fellowship for Regional Cooperation from the Conseil Régional de La Réunion. The authors wish to thank Nathalie Becker and anonymous reviewers for their comments, critical reviewing, and revising the English version of the manuscript.

Supplementary material

705_2013_1664_MOESM1_ESM.docx (16 kb)
Supplementary material 1 Nucleotide sequence annotation of cassava mosaic alphasatellite and the corresponding amino acid sequence of the Rep gene. The nucleotide sequences begin after the nick site () within the conserved nonanucleotide sequence TAGTATTAC (bold font) located in the stem-loop structure (highlighted in red). The TATA box (nucleotides 1453-1460, highlighted in blue) is located at the 5’ of the stem-loop, and the polyadenylation signal (nucleotides 990-995, highlight in green) is located at the 3’ of the Rep gene (highlighted in yellow). The predicted Rep amino acid sequence contains the tyrosine residue (amino acid 90, in bold) and the NTP-binding motif GGEGKS (amino acids 188-193, highlighted in purple) (DOCX 16 kb)
705_2013_1664_MOESM2_ESM.xlsx (10 kb)
Supplementary material 2 Description of cassava samples from the north of Madagascar (Diana region) in which cassava mosaic alphasatellite was detected by PCR (XLSX 10 kb)
705_2013_1664_MOESM3_ESM.xlsx (17 kb)
Supplementary material 3 Names, acronyms and GenBank/EMBL/DDBJ accession numbers of alphasatellites used in this study (XLSX 17 kb)

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Mireille Harimalala
    • 1
    • 2
    • 3
  • Alexandre De Bruyn
    • 1
    • 2
  • Murielle Hoareau
    • 1
  • Alice Andrianjaka
    • 3
  • Sahondramalala Ranomenjanahary
    • 4
  • Bernard Reynaud
    • 1
  • Pierre Lefeuvre
    • 1
  • Jean-Michel Lett
    • 1
    Email author
  1. 1.CIRAD, UMR PVBMT, Pôle de Protection des PlantesSaint-PierreFrance
  2. 2.Université de La Réunion, UMR PVBMT, Pôle de Protection des PlantesSaint-PierreFrance
  3. 3.Faculté des SciencesUniversité d’AntananarivoAntananarivoMadagascar
  4. 4.Laboratoire de Pathologie VégétaleFOFIFAAmbatobeMadagascar

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