Molecular characterization of a new alphasatellite associated with a cassava mosaic geminivirus in Madagascar
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Two complete nucleotide sequences of an alphasatellite isolated from a cassava plant with mosaic disease symptoms in Madagascar are described and analyzed. While the helper begomovirus was identified as an isolate of East African cassava mosaic Kenya virus (EACMKV), its associated alphasatellite was most closely related (80 % nucleotide sequence identity) to cotton leaf curl Gezira alphasatellite. These satellite molecules have typical features of alphasatellites, with a single gene in the virion sense, an A-rich region and a stem-loop structure. According to the proposed species demarcation threshold of alphasatellites (83 % nucleotide identity), they are isolates of a new species for which we propose the name “Cassava mosaic alphasatellite”.
KeywordsCassava Plant Cassava Mosaic Disease African Cassava Mosaic Virus Virion Sense Satellite Molecule
This study was funded by the European Union (FEDER), the Région Réunion and CIRAD. MH is a recipient of a PhD fellowship for Regional Cooperation from the Conseil Régional de La Réunion. The authors wish to thank Nathalie Becker and anonymous reviewers for their comments, critical reviewing, and revising the English version of the manuscript.
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