Establishment of an agroinoculation system for broad bean wilt virus 2
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We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5’ end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.
KeywordsCucumber Mosaic Virus Iwate Prefecture B935 Isolate Gentian Plant Eustoma Grandiflorum
We thank Kazue Obara for technical assistance, and Yasuya Iwadate and Kazumichi Fujiwara for collecting the gentian sample. This study was supported by the Iwate Prefectural Government.
Conflict of interest
The authors declare that they have no conflict of interest.
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