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Archives of Virology

, Volume 158, Issue 6, pp 1389–1392 | Cite as

Novel cyclovirus discovered in the Florida woods cockroach Eurycotis floridana (Walker)

  • Marco Padilla-Rodriguez
  • Karyna Rosario
  • Mya Breitbart
Annotated Sequence Record

Abstract

A novel cyclovirus (proposed genus “Cyclovirus”, family Circoviridae) was discovered in a specimen of Eurycotis floridana (Walker), also known as the Florida woods cockroach or palmetto bug, collected from Tarpon Springs, Florida. The Florida woods cockroach–associated cyclovirus GS140 (FWCasCyV-GS140) was obtained through a degenerate PCR assay and showed 64 % genome-wide pairwise identity to a cyclovirus identified in bat feces. This finding supports recent reports suggesting that Circoviridae members, traditionally thought to only infect vertebrates, are present within insect populations.

Keywords

Fecal Matter Insect Prey Alternative Donor Number JX569794 Accession Number JX569794 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors would like to thank Kelly Miller and Junior Girl Scout Troop 140 for assistance with insect collection. This research was funded by grants from the National Science Foundation (DEB-1025915 and DEB-1239976).

References

  1. 1.
    Todd D (2000) Circoviruses: immunosupressive threats in avian species: a review. Avian Pathol 29:373–394PubMedCrossRefGoogle Scholar
  2. 2.
    Rosario K, Duffy S, Breitbart M (2009) Diverse circovirus-like genome architectures revealed by environmental metagenomics. J Gen Virol 90:2418–2424PubMedCrossRefGoogle Scholar
  3. 3.
    Rosario K, Duffy S, Breitbart M (2012) A field guide to eukaryotic circular single-stranded DNA viruses: insights gained from metagenomics. Arch Virol 157:1851–1871PubMedCrossRefGoogle Scholar
  4. 4.
    Li L, Kapoor A, Slikas B, Bamidele OS, Wang C, Shaukat S, Masroor MA, Wilson ML, Ndjango JN, Peeters M, Gross-Camp ND, Muller MN, Hahn BH, Wolfe ND, Triki H, Bartkus J, Zaidi SZ, Delwart E (2010) Multiple diverse circoviruses infect farm animals and are commonly found in humans and chimpanzee feces. J Virol 84:1674–1682PubMedCrossRefGoogle Scholar
  5. 5.
    Li L, Shan T, Soji OB, Alam MM, Kunz TH, Zaidi SZ, Delwart E (2011) Possible cross-species transmission of circoviruses and cycloviruses among farm animals. J Gen Virol 92:768–772PubMedCrossRefGoogle Scholar
  6. 6.
    Rosario K, Marinov M, Stainton D, Kraberger S, Wiltshire EJ, Collings DA, Walters M, Martin DP, Breitbart M, Varsani A (2011) Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies. J Gen Virol 92:1302–1306PubMedCrossRefGoogle Scholar
  7. 7.
    Rosario K, Dayaram A, Marinov M, Ware J, Kraberger S, Stainton D, Breitbart M, Varsani A (2012) Diverse circular single-stranded DNA viruses discovered in dragonflies (Odonata: Epiprocta). J Gen Virol 93:2668–2681PubMedCrossRefGoogle Scholar
  8. 8.
    Ge XY, Li JL, Peng C, Wu LJ, Yang XL, Wu YQ, Zhang YZ, Shi ZL (2011) Genetic diversity of novel circular ssDNA viruses in bats in China. J Gen Virol 92:2646–2653PubMedCrossRefGoogle Scholar
  9. 9.
    Padgett RA, Burge CB (2005) Splice sites. eLS. doi: 10.1038/npg.els.0005044 Google Scholar
  10. 10.
    Bell WJ, Louis MR, Nalepa CA (2007) Cockroaches: ecology, behavior, and natural history. Johns Hopkins University Press, BaltimoreGoogle Scholar
  11. 11.
    Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  12. 12.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCrossRefGoogle Scholar
  13. 13.
    Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321PubMedCrossRefGoogle Scholar
  14. 14.
    Abascal F, Zardoya R, Posada D (2005) ProtTest: selection of best-fit models of protein evolution. Bioinformatics 21:2104–2105PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Marco Padilla-Rodriguez
    • 1
  • Karyna Rosario
    • 1
  • Mya Breitbart
    • 1
  1. 1.College of Marine ScienceUniversity of South FloridaSt. PetersburgUSA

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