Archives of Virology

, Volume 158, Issue 4, pp 753–763 | Cite as

Biological properties of bovine viral diarrhea virus quasispecies detected in the RK13 cell line

  • Mahmod Muhsen
  • Hiroshi Aoki
  • Hidetoshi Ikeda
  • Akio FukushoEmail author
Original Article


The rabbit kidney cell line RK13 has been reported to be contaminated with noncytopathogenic (ncp) bovine viral diarrhea virus (BVDV). Persistent infection was confirmed by demonstrating the stability of virus titers (104.6±0.5 TCID50/ml) and BVDV positive cells (71.9 ± 3.12 %), over six successive passages. Based on the “exaltation of Newcastle disease virus” (END) and reverse plaque formation methods, two types of ncp viruses were isolated, END-phenomenon-positive and negative. Isolates, RK13/E+ and RK13/E, demonstrated (1) differing levels of reproducibility in cell cultures, (2) similar antigenicity against BVDV antisera, (3) identical 5′-UTR region nucleotide sequences, (4) four amino acid differences throughout the genomic open reading frame, and (5) better growth ability in primary rabbit cells than other laboratory strains when inoculated in parallel at an MOI of 0.01. Overall, the BVDV population in RK13 cells consists of at least two different END characteristic quasispecies that are adapted to cultures of rabbit origin, giving rise to naturally attenuated BVDV strains that can be used in vaccine development.


Newcastle Disease Virus Bovine Viral Diarrhea Virus Vesicular Stomatitis Virus Classical Swine Fever Virus Interference Phenomenon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was partially supported by Japan International Cooperation Agency (JICA). The authors thank Dr. Peter Lee for his kind help with the preparation of the manuscript.


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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Mahmod Muhsen
    • 1
  • Hiroshi Aoki
    • 2
  • Hidetoshi Ikeda
    • 1
  • Akio Fukusho
    • 2
    Email author
  1. 1.Laboratory of Veterinary Hygiene, School of Veterinary Medicine, Faculty of Veterinary ScienceNippon Veterinary and Life Science UniversityTokyoJapan
  2. 2.Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary ScienceNippon Veterinary and Life Science UniversityTokyoJapan

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