Archives of Virology

, Volume 158, Issue 3, pp 685–689 | Cite as

Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A

  • C. J. Kasanga
  • T. Yamaguchi
  • H. M. Munang’andu
  • K. Ohya
  • H. Fukushi
Brief Report
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Abstract

We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.

Keywords

Deduce Amino Acid Sequence Vaccine Virus Genome Segment Nucleotide Sequence Identity Infectious Bursal Disease Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

We wish to thank the participating farmers in Zambia. This research was partly supported by Grants-in-Aid for Basic Scientific Research number (A) 17255010 and (C) 18580308 from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The Southern African Centre for Infectious Disease Surveillance is acknowledged for funding CJK to present some of the results detailed in this paper to the 1st One Health Conference in Africa held at the National Institute for Communicable Diseases, Johannesburg, South Africa, July 2011.

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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • C. J. Kasanga
    • 1
  • T. Yamaguchi
    • 2
  • H. M. Munang’andu
    • 3
  • K. Ohya
    • 4
  • H. Fukushi
    • 4
  1. 1.Department of Veterinary Microbiology and Parasitology, Molecular Biology and Biotechnology SectionSokoine University of AgricultureMorogoroTanzania
  2. 2.The Avian Zoonoses Center, Faculty of AgricultureTottori UniversityTottoriJapan
  3. 3.Department of Paraclinical Sciences, School of Veterinary MedicineUniversity of ZambiaLusakaZambia
  4. 4.Laboratory of Veterinary Microbiology, Faculty of Applied Biological SciencesGifu UniversityGifuJapan

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