Archives of Virology

, Volume 158, Issue 3, pp 549–558 | Cite as

Genetic diversity of porcine sapoviruses, kobuviruses, and astroviruses in asymptomatic pigs: an emerging new sapovirus GIII genotype

  • Lucie Dufkova
  • Ivana Scigalkova
  • Romana Moutelikova
  • Hana Malenovska
  • Jana Prodelalova
Original Article


Small, non-enveloped RNA viruses belonging to the genera Sapovirus, Kobuvirus, and Mamastrovirus are usually associated with gastroenteritis in humans and animals. These enteric pathogens are considered potential zoonotic agents. In this study, the prevalence and genetic diversity of sapoviruses (SaVs), kobuviruses (KoVs), and astroviruses (AstVs) in asymptomatic pigs were investigated using a PCR approach. KoV was found to be the most prevalent virus (87.3 %), followed by AstV (34.2 %) and SaV (10.2 %). Interestingly, the intra- and inter-cluster distances between porcine SaV capsid sequences revealed one strain (P38/11/CZ) that formed a new genotype within genogroup III of porcine SaVs, and it is tentatively called “P38/11-like” genotype. Moreover, this is the first report of porcine kobuvirus detection on Czech pig farms. The high prevalence rate of gastroenteritis-producing viruses in clinically healthy pigs represents a continuous source of infection of pigs, and possibly to humans.


Amino Acid Sequence Identity Porcine Strain Porcine SaVs GIII Genogroup Partial Capsid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Jindriska Bendova and Andrea Kyjovska for excellent technical assistance. This work was supported by the Ministry of Agriculture of the Czech Republic (Grant No. NAZV QH81061) and the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. AdmireVet CZ.1.05/2.1.00/01.006, ED006/01/01).

Conflict of interest

The authors declare that they have no actual or potential conflict of interest.


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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Lucie Dufkova
    • 1
  • Ivana Scigalkova
    • 2
  • Romana Moutelikova
    • 1
  • Hana Malenovska
    • 1
  • Jana Prodelalova
    • 1
  1. 1.Veterinary Research InstituteBrnoCzech Republic
  2. 2.Department of Experimental Biology, Faculty of ScienceMasaryk UniversityBrnoCzech Republic

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