Archives of Virology

, Volume 157, Issue 12, pp 2447–2452 | Cite as

Caladenia virus A, an unusual new member of the family Potyviridae from terrestrial orchids in Western Australia

  • Stephen J. Wylie
  • Aaron J. Y. Tan
  • Hua Li
  • Kingsley W. Dixon
  • Michael G. K. Jones
Brief Report


An isolate of a new virus, Caladenia virus A (CalVA), was identified infecting Australian terrestrial orchids. The complete genome of 9,847 nucleotides encodes 11 gene products typical of most members of the family Potyviridae. Sequence comparisons of the polyprotein revealed that CalVA shared highest sequence identity (37.5-39.6 %) with members of the genus Poacevirus. Although a vector for CalVA was not identified, a mite transmission motif was present in the helper component protease, indicating that, like other poaceviruses, mites may transmit it. CalVA is the only proposed member of the genus Poacevirus not isolated from a poaceous host.


  1. 1.
    Adams MJ, Carstens EB (2012) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses. Arch Virol 157:1411–1422PubMedCrossRefGoogle Scholar
  2. 2.
    Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479PubMedCrossRefGoogle Scholar
  3. 3.
    Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487PubMedCrossRefGoogle Scholar
  4. 4.
    Blanc S, Lopezmoya JJ, Wang RY, Garcialampasona S, Thornbury DW, Pirone TP (1997) A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. Virology 231:141–147PubMedCrossRefGoogle Scholar
  5. 5.
    Chung BY-W, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. PNAS 105:5897–5902PubMedCrossRefGoogle Scholar
  6. 6.
    Drummond AJ, Ashton B, Cheung M, Heled J, Kearse M, Moir R, Stones-Havas S, Thierer T, Wilson A (2011) Geneious v5.5,
  7. 7.
    Gibbs A, Mackenzie A, Blanchfield AL, Cross P, Wilson CR, Kitajima E, Nightingale M, Clements M (2000) Viruses of orchids in Australia; their identification, biology and control. Austral Orchid Rev 65. Lewisham, NSW, AustraliaGoogle Scholar
  8. 8.
    Gibbs AJ, Mackenzie AM, Wei KJ, Gibbs MJ (2008) The potyviruses of Australia. Arch Virol 153:1411–1420PubMedCrossRefGoogle Scholar
  9. 9.
    Hall JS, Adams B, Parsons TJ, French R, Lane LC, Jensen SG (1998) Molecular cloning, sequencing and phylogenetic relationships of a new potyvirus: sugarcane streak mosaic virus, and a reevaluation of the classification of the Potyviridae. Mol Phylogenet Evol 10:323–332PubMedCrossRefGoogle Scholar
  10. 10.
    Hema M, Sreenivasulu P, Savithri HS (2002) Taxonomic position of sugarcane streak mosaic virus in the family Potyviridae. Arch Virol 147:1997–2007PubMedCrossRefGoogle Scholar
  11. 11.
    Hoffman N, Brown A (2011) Orchids of south-west Australia, 3rd edn. Hoffman, PerthGoogle Scholar
  12. 12.
    Hopper SD, Gioia P (2004) The southwest Australian floristic region: evolution and conservation of a global hot spot of biodiversity. Ann Rev Ecol Evol Syst 35:623–650CrossRefGoogle Scholar
  13. 13.
    King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, LondonGoogle Scholar
  14. 14.
    Koonin EV (1991) The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72:2197–2206PubMedCrossRefGoogle Scholar
  15. 15.
    Li W, Zhen He Z, Li S, Huang Y, Zhang Z, Jiang D, Wang X, Luo Z (2011) Molecular characterization of a new strain of sugarcane streak mosaic virus (SCSMV). Arch Virol 156:2101–2104PubMedCrossRefGoogle Scholar
  16. 16.
    Mackenzie AM, Nolan M, Wei K-J, Clements MA, Gowanlock D, Wallace BJ, Gibbs AJ (1998) Ceratobium mosaic potyvirus: another virus from orchids. Arch Virol 143:903–914PubMedCrossRefGoogle Scholar
  17. 17.
    Pirone TP (1991) Virus genes and gene products that determine insect transmissibility. Semin Virol 2:81–87Google Scholar
  18. 18.
    Rabenstein F, Maiss E, French R (2010) Characterization of novel potyviruses occurring in grasses of the genus Festuca and Dactylis in Germany. Abstract. Deutsche Pflanzenschutztagung, Berlin, Germany, 6–9 September, 2010. Julius-Kühn-Archiv 428:332–333Google Scholar
  19. 19.
    Roossinck MJ, Schneider W (2006) Mutant clouds and occupation of sequence space in plant RNA viruses. Curr Top Microbiol Immunol 299:337–348PubMedCrossRefGoogle Scholar
  20. 20.
    Seifers DL, Martin TL, Harvey TL, Fellers JP, Michaud JP (2009) Identification of the wheat curl mite as the vector of Triticum mosaic virus. Plant Dis 93:25–29CrossRefGoogle Scholar
  21. 21.
    Stenger DC, Hein GL, Gildow FE, Horken KM, French R (2005) Plant virus HC-Pro is a determinant of eriophyid mite transmission. J Virol 79:9054–9061PubMedCrossRefGoogle Scholar
  22. 22.
    Swarts ND, Dixon KW (2009) Terrestrial orchid conservation in the age of extinction. Ann Bot 104:543–556PubMedCrossRefGoogle Scholar
  23. 23.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCrossRefGoogle Scholar
  24. 24.
    Tatinemi A, Ziems AD, Wegulo SN, French R (2009) Triticum mosaic virus: a distinct member of the family Potyviridae with an unusually long leader sequence. Phytopathol 99:943–950CrossRefGoogle Scholar
  25. 25.
    Young BA, Hein GL, French R, Stenger DC (2007) Substitution of conserved cysteine residues in wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite. Arch Virol 152:2107–2111PubMedCrossRefGoogle Scholar
  26. 26.
    Viswanathan R, Balamuralikrishnan M, Karuppaiah R (2008) Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane. Virus Gene 36:553–564CrossRefGoogle Scholar
  27. 27.
    Webster C, Jones RAC, Coutts BA, Jones MGK, Wylie SJ (2007) Virus impact at the interface of an ancient ecosystem and a recent agroecosystem: studies on three legume-infecting potyviruses in the southwest Australian floristic region. Plant Pathol 56:729–742CrossRefGoogle Scholar
  28. 28.
    Wylie SJ, Luo H, Li H, Jones MGK (2012) Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples. Arch Virol 157:271–284PubMedCrossRefGoogle Scholar
  29. 29.
    Xu DL, Zhou GH, Xie YJ, Mock R, Li R (2010) Complete nucleotide sequence and taxonomy of sugarcane streak mosaic virus, member of a novel genus in the family Potyviridae. Virus Gene 40:432–439CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Stephen J. Wylie
    • 1
  • Aaron J. Y. Tan
    • 1
  • Hua Li
    • 1
  • Kingsley W. Dixon
    • 2
  • Michael G. K. Jones
    • 1
  1. 1.Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and BiotechnologyMurdoch UniversityPerthAustralia
  2. 2.Botanic Gardens and Parks AuthorityKings Park and Botanic GardenWest PerthAustralia

Personalised recommendations