Archives of Virology

, Volume 157, Issue 12, pp 2431–2435 | Cite as

Bacteriophage vB_EcoM_FV3: a new member of “rV5-like viruses”

  • Lidija Truncaite
  • Eugenijus Šimoliūnas
  • Aurelija Zajančkauskaite
  • Laura Kaliniene
  • Roma Mankevičiūte
  • Juozas Staniulis
  • Vytautas Klausa
  • Rolandas Meškys
Brief Report

Abstract

A proposed new genus of the family Myoviridae, “rV5-like viruses”, includes two lytic bacteriophages: Escherichia coli O157: H7-specific bacteriophage rV5 and Salmonella phage PVP-SE1. Here, we present basic properties and genomic characterization of a novel rV5-like phage, vB_EcoM_FV3, which infects E. coli K-12-derived laboratory strains and replicates at high temperature (up to 47 °C). The 136,947-bp genome of vB_EcoM_FV3 contains 218 open reading frames and encodes 5 tRNAs. The genomic content and organization of vB_EcoM_FV3 is more similar to that of rV5 than to PVP-SE1, but all three phages share similar morphological characteristics and form a homogeneous phage group.

Keywords

Escherichia coli rV5-like viruses Bacteriophage vB_EcoM_FV3 High temperature 

Notes

Acknowledgments

We thank Dr. Ken Kreuzer, Dr. Victor Krylov and Dr. Lindsay W. Black for E. coli strains. This work was supported in part by the Lithuanian State Science and Studies Foundation (Grant No. N-07005) and was also funded by a grant (No. MIP-76/2010) from the Research Council of Lithuania.

Supplementary material

705_2012_1449_MOESM1_ESM.doc (286 kb)
Supplementary material 1 Table 1S. A list of bacteriophage vB_EcoM_FV3 putative genes with predicted functions. (DOC 285 kb)
705_2012_1449_MOESM2_ESM.tif (4.6 mb)
Supplementary material 2 Fig. 1S. Functional genomic map of bacteriophage vB_EcoM_FV3. Putative genes with predicted coding functions are represented as arrows. The colour code for the online version of the article is as follows: yellow—DNA replication, recombination, repair and packaging; brown—transcription, translation, nucleotide metabolism; blue—virion structure; green—chaperones/assembly; purple—lysis, host or phage interactions; grey—ORFs of unknown function; red—unique FV3 ORFs of unknown function; black—tRNA. (TIFF 4748 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Lidija Truncaite
    • 1
  • Eugenijus Šimoliūnas
    • 1
  • Aurelija Zajančkauskaite
    • 1
  • Laura Kaliniene
    • 1
  • Roma Mankevičiūte
    • 1
  • Juozas Staniulis
    • 2
  • Vytautas Klausa
    • 1
  • Rolandas Meškys
    • 1
  1. 1.Department of Molecular Microbiology and BiotechnologyInstitute of Biochemistry of Vilnius UniversityVilniusLithuania
  2. 2.Laboratory of Plant VirusesInstitute of Botany of the Nature Research CentreVilniusLithuania

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