Archives of Virology

, Volume 157, Issue 10, pp 2027–2030 | Cite as

A novel cassava-infecting begomovirus from Madagascar: cassava mosaic Madagascar virus

  • Mireille Harimalala
  • Pierre Lefeuvre
  • Alexandre De Bruyn
  • Fidèle Tiendrébéogo
  • Murielle Hoareau
  • Julie Villemot
  • Sahondramalala Ranomenjanahary
  • Alice Andrianjaka
  • Bernard Reynaud
  • Jean-Michel LettEmail author
Annotated Sequence Record


Cassava mosaic geminiviruses (CMGs) are implicated in cassava mosaic disease (CMD), the main constraint to cassava production in Africa. Here, we report the complete nucleotide sequences of the DNA-A and DNA-B of a newly characterized CMG found infecting cassava in Madagascar, for which we propose the tentative name cassava mosaic Madagascar virus. With the exception of two recombinant regions that resembled a CMG, we determined that the non-recombinant part of the DNA-A component is distantly related to the other CMGs. Whereas the DNA-B component possesses one recombinant region originating from an unidentified virus, the rest of the genome was seen to be closely related to members of the species East African cassava mosaic Zanzibar virus (EACMZV). Phylogenetic analysis based on complete genome sequences demonstrated that DNA-A and DNA-B components are outliers related to the clade of EACMV-like viruses and that DNA-A is related to the monopartite tomato leaf curl begomoviruses described in islands in the south-west Indian Ocean.


Tomato Leaf Recombinant Region Cassava Mosaic Disease Recombinant Fragment Cassava Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was funded by the European Union (FEDER), the Région Réunion and CIRAD. MH is a recipient of a PhD fellowship for Regional Cooperation from the Conseil Régional de La Réunion.

Supplementary material

705_2012_1399_MOESM1_ESM.eps (117 kb)
Supplementary Fig. 1 Maximum-likelihood (ML) trees showing phylogenetic relationships between the DNA-B component of CMMGV and those of the other representative African and Indian CMGs. Values at nodes indicate the percentage of bootstrap support (1000 replicates; only bootstrap values greater than 70 % are shown). ML trees inferred from alignments of (A) recombinant region c (positions 2367 to 2698) and (B) the non-recombinant region. For begomovirus acronyms and accession numbers, see Supplementary Table 2 (EPS 117 kb)
705_2012_1399_MOESM2_ESM.xls (29 kb)
Supplementary material 2 (XLS 29 kb)
705_2012_1399_MOESM3_ESM.xls (37 kb)
Supplementary material 3 (XLS 37 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Mireille Harimalala
    • 1
    • 2
    • 3
  • Pierre Lefeuvre
    • 1
  • Alexandre De Bruyn
    • 1
    • 2
  • Fidèle Tiendrébéogo
    • 4
  • Murielle Hoareau
    • 1
  • Julie Villemot
    • 1
  • Sahondramalala Ranomenjanahary
    • 5
  • Alice Andrianjaka
    • 3
  • Bernard Reynaud
    • 1
  • Jean-Michel Lett
    • 1
    Email author
  1. 1.UMR PVBMT, Pôle de Protection des PlantesCIRADSaint-PierreFrance
  2. 2.UMR PVBMT, Pôle de Protection des PlantesUniversité de La RéunionSaint-PierreFrance
  3. 3.Faculté des SciencesUniversité d’AntananarivoAntananarivoMadagascar
  4. 4.INERAOuagadougou 01Burkina Faso
  5. 5.Laboratoire de Pathologie VégétaleFOFIFAAmbatobeMadagascar

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