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Archives of Virology

, Volume 157, Issue 9, pp 1779–1783 | Cite as

Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of a new SFTS bunyavirus

  • Guoliang Yang
  • Bing Li
  • Lanzheng Liu
  • Weichu Huang
  • Wen Zhang
  • Yuandong Liu
Brief Report

Abstract

The etiological agent of severe fever with thrombocytopenia syndrome (SFTS) is a bunyavirus that was first identified in China in 2009. We have developed and validated a one-step, single-tube, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SFTS bunyavirus (SFTSV). This assay demonstrated high specificity and sensitivity, with a detection limit of 101 TCID50 ml−1. When combined with the fluorescent detection reagent (FDR) method, results could be determined by observing a color change within 30 min. As an accurate, rapid, simple and low-cost diagnostic method, this RT-LAMP assay will be helpful for detecting and preventing further SFTSV infection in China.

Keywords

Clinical diagnosis Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) Reverse transcription loop-mediated isothermal amplification (RT-LAMP) Family Bunyaviridae 

Supplementary material

705_2012_1348_MOESM1_ESM.pdf (55 kb)
Supplementary material 1 (PDF 55 kb)
705_2012_1348_MOESM2_ESM.pdf (63 kb)
Supplementary material 2 (PDF 63 kb)
705_2012_1348_MOESM3_ESM.pdf (19 kb)
Supplementary material 3 (PDF 19.1 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Guoliang Yang
    • 1
  • Bing Li
    • 2
  • Lanzheng Liu
    • 1
  • Weichu Huang
    • 2
  • Wen Zhang
    • 1
  • Yuandong Liu
    • 2
  1. 1.Jinan Center for Disease Control and PreventionJinanChina
  2. 2.Jinan Junqu Center for Disease Control and PreventionJinanChina

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