Archives of Virology

, Volume 157, Issue 8, pp 1611–1616 | Cite as

Genetic reassortment between Sathuperi and Shamonda viruses of the genus Orthobunyavirus in nature: implications for their genetic relationship to Schmallenberg virus

  • Tohru YanaseEmail author
  • Tomoko Kato
  • Maki Aizawa
  • Yozo Shuto
  • Hiroaki Shirafuji
  • Makoto Yamakawa
  • Tomoyuki Tsuda
Rapid Communication


The recent outbreak of malformations in ruminants in Northern Europe caused by Schmallenberg virus induced us to analyze the genetic properties of the related orthobunyaviruses and clarify their relationship. The sequencing of three genomic RNA segments of Sathuperi, Shamonda and Douglas viruses (SATV, SHAV and DOUV) revealed that the M RNA segment of SATV and DOUV had a high degree of sequence identity with that of Schmallenberg virus, but the S and L RNA segments closely matched those of SHAV. Phylogenetic analysis of the three genomic RNA segments indicated that Schmallenberg virus is a reassortant, with the M RNA segment from SATV and the S and L RNA segments from SHAV.


Reverse Transcription Polymerase Chain Reaction Nucleocapsid Protein Genetic Reassortment Akabane Virus Simbu Serogroup 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Mr. Hirotaka Horiwaki for excellent technical assistance. This work was supported by a fund from the National Agriculture and Food Resource Organization, NARO, Japan.

Supplementary material

705_2012_1341_MOESM1_ESM.doc (221 kb)
Supplement Fig 1 (DOC 221 kb)
705_2012_1341_MOESM2_ESM.doc (34 kb)
Supplement Table S1 (DOC 34 kb)
705_2012_1341_MOESM3_ESM.doc (54 kb)
Supplement Table S2 (DOC 54 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Tohru Yanase
    • 1
    Email author
  • Tomoko Kato
    • 1
  • Maki Aizawa
    • 2
  • Yozo Shuto
    • 3
  • Hiroaki Shirafuji
    • 1
  • Makoto Yamakawa
    • 1
  • Tomoyuki Tsuda
    • 4
  1. 1.Kyushu Research StationNational Institute of Animal Health, NAROKagoshimaJapan
  2. 2.Okinawa Prefectural Institute of Animal HealthNahaJapan
  3. 3.Oita Livestock Hygiene Service CenterOitaJapan
  4. 4.National Institute of Animal Health, NAROTsukubaJapan

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