Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization
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A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 104 TCID50. Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.
KeywordsVesicular Stomatitis Virus Envelope Glycoprotein Live Virus ELISA System Nipah Virus
We are grateful to Dr. K. Komase (Department of Virology III, National Institute of Infectious Diseases) for providing inactivated measles virus antigens. A part of this study was supported by a grant for Research on Emerging and Reemerging Infectious Diseases (H20-Shinkou-Ippan-006) from the Ministry of Health, Labor and Welfare of Japan.
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