Archives of Virology

, Volume 157, Issue 8, pp 1559–1564 | Cite as

Sequencing and analysis of the complete genome of Rana grylio virus (RGV)

  • Xiao-Ying Lei
  • Tong Ou
  • Ruo-Lin Zhu
  • Qi-Ya Zhang
Brief Report


Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.


Rock Bream Rana Grylio Virus Lymphocystis Disease Virus Tiger Frog Virus miRNA Code 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was funded by National Major Basic Research Program (2010CB126303, 2009CB118704), Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-Z-3), National Natural Science Foundation of China (31072239), and an FEBL research grant (Y15B121F01).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Xiao-Ying Lei
    • 1
  • Tong Ou
    • 1
  • Ruo-Lin Zhu
    • 1
  • Qi-Ya Zhang
    • 1
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesWuhanChina

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