Characterization and analysis of the full-length genome of a strain of the European QX-like genotype of infectious bronchitis virus
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In recent years, strains of infectious bronchitis virus belonging to the QX-like genotype have been causing huge economic losses in commercial chicken flocks in different countries in Europe. In order to expand the knowledge of the molecular features of these viruses, we have sequenced and characterized the complete genome of European QX-like IBV strain CK/SWE/0658946/10, which was isolated in 2010 in Sweden. The genome is 27664 nucleotides in length, comprising six genes and 5′ and 3′ untranslated regions. The ORF1a, spike and nucleocapsid genes were under strong positive selective pressure that resulted in genetic diversity in relation to classical IBV isolates. The full-length genome of the CK/SWE/0658946/10 strain has the highest nucleotide sequence identity (93.18%) to ITA/90254/2005 and the lowest nucleotide identity (89.10%) to strain CQ04-1. Phylogenetic analysis of partial S1 gene sequences of IBV strains showed that the European QX-like genotype comprises strains that have been predominantly circulating in this continent for the past decade.
KeywordsInfectious Bronchitis Virus Infectious Bronchitis Virus Strain Replicase Gene Chicken Flock Infectious Bronchitis Virus Isolate
This study was funded by Grant 70031/2009 of the National Veterinary Institute (SVA) Research Fund. We are also grateful to Ulla Berglöf and Karin von Schantz (SVA) for providing specific-pathogen-free eggs.
- 2.Enjuanes L, Brian D, Cavanagh D, Holmes K, Lai MMC, Laude H, Masters P, Rottier P, Siddell S et al (2000) Coronaviridae. In: Van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Lemon SM, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds) Virus taxonomy: seventh report of the international committee on taxonomy of viruses. Academic Press, New York, pp 835–849Google Scholar
- 10.Abro SH, Renstrom LH, Ullman K, Isaksson M, Zohari S, Jansson DS, Belak S, Baule C (2011) Emergence of novel strains of avian infectious bronchitis virus in Sweden. Vet microbiol 55(1):155–161Google Scholar