Archives of Virology

, Volume 157, Issue 6, pp 1093–1099 | Cite as

A novel totivirus-like virus isolated from bat guano

  • Xinglou Yang
  • Yunzhi Zhang
  • Xingyi Ge
  • Junfa Yuan
  • Zhengli Shi
Original Article

Abstract

Previous metagenomic analysis indicated that numerous insect viruses exist in bat guano. In this study, we isolated a novel double-stranded RNA virus, a tentative member of the family Totiviridae, designated Tianjin totivirus (ToV-TJ), from bat feces. The virus is an icosahedral particle with a diameter of 40-43 nm, and it causes cytopathic effect in Sf9, Hz, and C6/36 cell lines. Full-length genomic sequence analysis showed that ToV-TJ shares high similarity with the totivirus OMRV-AK4, which was recently isolated from mosquitoes in Japan. The full-length genome of the ToV-TJ was 7611 bp and contained two predicted non-overlapping open reading frames (ORFs): ORF1, encoding the capsid protein (CP), and ORF2, encoding an RNA-dependent RNA polymerase. Bioassay of ToV-TJ by feeding on the larvae of Spodoptera exigua and Helicoverpa armigera (Hubner) suggests that this virus is not infectious for these two larvae in vivo. Sequences similar to that of ToV-TJ have been detected in bat feces sampled in Yunnan and Hainan Provinces, suggesting that this virus is widely distributed.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Xinglou Yang
    • 1
  • Yunzhi Zhang
    • 2
  • Xingyi Ge
    • 1
  • Junfa Yuan
    • 3
  • Zhengli Shi
    • 1
  1. 1.State Key Laboratory of VirologyWuhan Institute of Virology, Chinese Academy of SciencesWuhanChina
  2. 2.Yunnan Institute of Endemic Diseases Control and PreventionDaliChina
  3. 3.College of Fisheries, Huazhong Agriculture UniversityWuhanChina

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