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Archives of Virology

, Volume 157, Issue 5, pp 993–998 | Cite as

Comparative efficacy of double-stranded RNAs targeting WSSV structural and nonstructural genes in controlling viral multiplication in Penaeus monodon

  • M. Sanjuktha
  • V. Stalin Raj
  • K. Aravindan
  • S. V. Alavandi
  • M. Poornima
  • T. C. SantiagoEmail author
Brief Report

Abstract

RNA interference (RNAi) is a potential strategy to control shrimp viral diseases, including the white spot disease caused by White Spot Syndrome Virus (WSSV). Selection of genes for targeting is an important criterion. We have compared the efficacy of dsRNAs targeting structural (vp28 and vp281) and nonstructural genes (rr1 and dnapol) of WSSV in controlling viral multiplication in Penaeus monodon. Targeting the rr1 and vp28 genes provided better protection (93.3% and 90% survival respectively) compared to vp281 and dnapol in experimentally infected shrimp. Temporal transcriptional analysis of the corresponding genes and PCR-based diagnosis of WSSV in samples collected at different time points in the experiment supported this observation, thereby indicating that targeting a combination of rr1 and vp28 would be effective in limiting WSSV multiplication.

Keywords

White Spot Syndrome Virus Positive Control Group Viral Life Cycle White Spot Syndrome Virus Infection Major Envelope Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Sanjuktha M acknowledges the financial support of the Council of Scientific and Industrial Research (CSIR) during her tenure as a CSIR Senior Research Fellow. The authors are thankful to the Director, CIBA, for his support. The authors are thankful to Dr. C. P. Balasubramanian for his help in performing the statistical analyses.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • M. Sanjuktha
    • 1
  • V. Stalin Raj
    • 1
    • 2
  • K. Aravindan
    • 1
  • S. V. Alavandi
    • 1
  • M. Poornima
    • 1
  • T. C. Santiago
    • 1
    • 3
    Email author
  1. 1.Aquatic Animal Health and Environment DivisionCentral Institute of Brackishwater AquacultureChennaiIndia
  2. 2.Department of VirologyRotterdamThe Netherlands
  3. 3.Entomology Research InstituteLoyola CollegeChennaiIndia

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