Comparative efficacy of double-stranded RNAs targeting WSSV structural and nonstructural genes in controlling viral multiplication in Penaeus monodon
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RNA interference (RNAi) is a potential strategy to control shrimp viral diseases, including the white spot disease caused by White Spot Syndrome Virus (WSSV). Selection of genes for targeting is an important criterion. We have compared the efficacy of dsRNAs targeting structural (vp28 and vp281) and nonstructural genes (rr1 and dnapol) of WSSV in controlling viral multiplication in Penaeus monodon. Targeting the rr1 and vp28 genes provided better protection (93.3% and 90% survival respectively) compared to vp281 and dnapol in experimentally infected shrimp. Temporal transcriptional analysis of the corresponding genes and PCR-based diagnosis of WSSV in samples collected at different time points in the experiment supported this observation, thereby indicating that targeting a combination of rr1 and vp28 would be effective in limiting WSSV multiplication.
KeywordsWhite Spot Syndrome Virus Positive Control Group Viral Life Cycle White Spot Syndrome Virus Infection Major Envelope Protein
Sanjuktha M acknowledges the financial support of the Council of Scientific and Industrial Research (CSIR) during her tenure as a CSIR Senior Research Fellow. The authors are thankful to the Director, CIBA, for his support. The authors are thankful to Dr. C. P. Balasubramanian for his help in performing the statistical analyses.
- 1.Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) (2005) Virus taxonomy, VIIIth report of the ICTV. Elsevier/Academic Press, LondonGoogle Scholar
- 2.Lightner DV (1996) A handbook of pathology and diagnostic procedures for diseases of penaeid shrimp. World Aquaculture Society, Baton RougeGoogle Scholar
- 6.Caipang CM, Verjan N, Ooi EL, Kondo H, Hirono I, Aoki T, Kiyono H, Yuki Y (2008) Enhanced survival of shrimp, Penaeus (Marsupenaeus) japonicus from white spot syndrome disease after oral administration of recombinant VP28 expressed in Brevibacillus brevis. Fish Shellfish Immunol 25:315–320PubMedCrossRefGoogle Scholar
- 16.Robalino J, Bartlett T, Shepard E, Prior S, Jaramillo G, Scura E, Chapman RW, Gross PS, Browdy CL, Warr GW (2005) Double-starnded RNA induces sequence-specific antiviral silencing in addition to nonspecific immunity in a marine shrimp: convergence of RNA interference and innate immunity in the invertebrate antiviral response? J Virol 79:13561–13571PubMedCrossRefGoogle Scholar
- 21.Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- 23.Lin ST, Tzeng HF, Lo CF, Kou GH (2004) White spot syndrome virus (WSSV) ribonucleotide reductase (RR) is differentiated from the cellular RRs by its insensitivity to allosteric inhibition by dTTP and dATP. J Fish Soc Taiwan 31:191–200Google Scholar