Archives of Virology

, Volume 157, Issue 4, pp 669–679 | Cite as

In vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection

  • Guofeng Zhang
  • Feng Zhou
  • Bin Gu
  • Chuanling Ding
  • Dongju Feng
  • Fangyi Xie
  • Jinfeng Wang
  • Chun Zhang
  • Qingxian Cao
  • Yinlai Deng
  • Weixing HuEmail author
  • Kun YaoEmail author
Original Article


Enterovirus 71(EV71) causes recurring outbreaks of hand, foot and mouth disease and encephalitis leading to complications or death in young children. More effective antiviral drugs are needed to prevent or reduce EV71-related disease and complications. However, there are no standard models currently in use to evaluate activity against EV71 infection both in vitro and in vivo. In this study, the activity of ribavirin and pleconaril against EV71 infection was evaluated in two models. An in vitro EV71 infection model was developed in RD cells, and an in vivo EV71 infection model was applied. Ribavirin and pleconaril effectively increased the viability of infected cells. Pleconaril reduced the morbidity and mortality of one-day-old infected mice, but ribavirin did not protect the infected mice. In all, the results demonstrated that infected cells and infected mice can be used to evaluate antiviral activity of ribavirin and pleconaril against EV71 infection in vitro and in vivo.


Antiviral Activity Infected Mouse EV71 Infection Spinal Ganglion Lassa Fever 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the National Science and Technology Major Project of China (NO.2009zx09103-005), Professor Zhiyang Shi, who kindly provided virus from the Key Lab of Enteric Pathogenic Microbiology of the Chinese Ministry of Health, and Qingxian Cao and Yinlai Deng from the Jiangsu Wu Zhong Group.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Guofeng Zhang
    • 1
    • 2
  • Feng Zhou
    • 1
  • Bin Gu
    • 1
    • 2
  • Chuanling Ding
    • 3
  • Dongju Feng
    • 1
  • Fangyi Xie
    • 1
  • Jinfeng Wang
    • 1
  • Chun Zhang
    • 1
    • 2
  • Qingxian Cao
    • 4
  • Yinlai Deng
    • 4
  • Weixing Hu
    • 2
    Email author
  • Kun Yao
    • 1
    Email author
  1. 1.Department of Microbiology and ImmunityNanjing Medical UniversityNanjingChina
  2. 2.Department of NeurosurgeryThe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina
  3. 3.Tumor Immunolobiology Program, James Graham Brown Cancer CenterUniversity of LouisvilleLouisvilleUSA
  4. 4.Jiangsu Wuzhong GroupSuzhouChina

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