In vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection
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Enterovirus 71(EV71) causes recurring outbreaks of hand, foot and mouth disease and encephalitis leading to complications or death in young children. More effective antiviral drugs are needed to prevent or reduce EV71-related disease and complications. However, there are no standard models currently in use to evaluate activity against EV71 infection both in vitro and in vivo. In this study, the activity of ribavirin and pleconaril against EV71 infection was evaluated in two models. An in vitro EV71 infection model was developed in RD cells, and an in vivo EV71 infection model was applied. Ribavirin and pleconaril effectively increased the viability of infected cells. Pleconaril reduced the morbidity and mortality of one-day-old infected mice, but ribavirin did not protect the infected mice. In all, the results demonstrated that infected cells and infected mice can be used to evaluate antiviral activity of ribavirin and pleconaril against EV71 infection in vitro and in vivo.
KeywordsAntiviral Activity Infected Mouse EV71 Infection Spinal Ganglion Lassa Fever
This work was supported by the National Science and Technology Major Project of China (NO.2009zx09103-005), Professor Zhiyang Shi, who kindly provided virus from the Key Lab of Enteric Pathogenic Microbiology of the Chinese Ministry of Health, and Qingxian Cao and Yinlai Deng from the Jiangsu Wu Zhong Group.
- 10.Chen SP, Huang YC, Li WC, Chiu CH, Huang CG, Tsao KC, Lin TY (2010) Comparison of clinical features between coxsackievirus A2 and enterovirus 71 during the enterovirus outbreak in Taiwan, 2008: a children’s hospital experience. J Microbiol Immunol 43:99–104Google Scholar
- 20.Kamar N, Rostaing L, Abravanel F, Garrouste C, Lhomme S, Esposito L, Basse G, Cointault O, Ribes D, Nogier MB, Alric L, Peron JM, Izopet J (2010) Ribavirin therapy inhibits viral replication on patients with chronic hepatitis e virus infection. Gastroenterology 139:1612–1618PubMedCrossRefGoogle Scholar
- 22.Hsu HW, Wang JH, Liu CL, Chen CS, Wang YC (2011) Anti-enterovirus 71 activity screening of Taiwanese folk medicinal plants and immune modulation of Ampelopsis brevipedunculata (Maxim.) Trautv against the virus. Afr J Microbiol Res 5:2500–2511Google Scholar