Genetic characterization of Pseudomonas aeruginosa bacteriophage KPP10
- 424 Downloads
Bacteriophage (phage) KPP10 has been used in experimental phage therapies directed against P. aeruginosa infections. To examine the eligibility of phage KPP10 as a therapeutic phage, its genome was analyzed. The genomic DNA was shown to be 88,322 bp long, with 158 open reading frames (ORFs), and three tRNA genes were predicted. No ORF-encoded pathogenicity or lysogenization factor was predicted. A comparative genomic analysis revealed that phage KPP10, together with phage PAK_P3, can be grouped as a new type of lytic phage infecting P. aeruginosa. Phage KPP10 is considered to be suitable for therapeutic purposes because it is a lytic phage without ORF-encoded pathogenicity or a lysogenization factors.
KeywordsPseudomonas aeruginosa Bacteriophage Therapeutic phage Genomes
We thank Mr. Shingo Sakurai for his technical support, and thank the Science Research Center, Kochi, Japan, for its experimental support. This study was funded by a Grant-in-Aid for Research Activity Startup (no. 22890129), Kochi System Glycobiology Center, Kochi, Japan, and the Center of Biomembrane Functions Controlling Biological Systems, Kochi, Japan.
- 3.Black LW, Showe MK, Steven AC (1994) Morphogenesis of the T4 head. In: Eiserling FA, Black LW (eds) Molecular biology of bacteriophage T4. American Society for Microbiology, Washington, pp 218–258Google Scholar
- 23.Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar