Archives of Virology

, Volume 157, Issue 2, pp 397–400 | Cite as

Complete genome sequence of a banana bract mosaic virus isolate infecting the French plantain cv. Nendran in India

  • V. Balasubramanian
  • R. Selvarajan
Annotated Sequence Record


The first complete genome sequence of an Indian isolate (TRY) of Banana bract mosaic virus (BBrMV) was determined following virus RNA extraction from the French plantain cv. Nendran (AAB). The complete genome was 9711 nucleotides excluding the poly(A) tail and had a genome organization similar to that of a Philippine (PHI) isolate characterized earlier. When compared to BBrMV-PHI, the complete genome sequence of BBrMV-TRY was 94% identical at the nucleotide level and its ten mature proteins had amino acid sequence identities ranging from 88 to 98%. Phylogenetic analysis suggests that the BBrMV-TRY isolate is closely related to the BBrMV-PHI isolate.


Complete Genome Sequence Amino Acid Sequence Identity Coat Protein Gene Aphid Transmission Putative Cleavage Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are thankful to the Director of the National Research Centre for Banana (NRCB), Tiruchirapalli, T.N (India), for providing the necessary facilities. The Indian Council of Agriculture Research (ICAR) Project No.16 (20/2004-Hort.II) under the network project on “Diagnosis of emerging plant viruses” is duly acknowledged for financial support.


  1. 1.
    Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487PubMedCrossRefGoogle Scholar
  2. 2.
    Ha C, Coombs S, Revil PA, Harding RM, Vu M, Dale JL (2008) Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. Arch Virol 153:25–36PubMedCrossRefGoogle Scholar
  3. 3.
    Magnaye LV, Espino RRC (1990) Note: Banana bract mosaic, a new disease of banana I. Symptomatology. Philippine Agric 73:55–59Google Scholar
  4. 4.
    Rodoni BC, Ahlawat YS, Varma A, Dale JL, Harding RM (1997) Identification and characterization of banana bract mosaic virus in India. Plant Dis 81:669–672CrossRefGoogle Scholar
  5. 5.
    Rodoni BC, Dale JL, Harding RM (1999) Characterization and expression of the coat protein-coding region of banana bract mosaic potyvirus, development of diagnostic assays and detection of the virus in banana plants from five countries in Southeast Asia. Arch Virol 144:1725–1737PubMedCrossRefGoogle Scholar
  6. 6.
    Roperos NI, Magnaye LV (1991) Status of banana diseases in Asia and the Pacific. In: Valmayor R (ed) International network for the improvement of banana and plantain. Montpellier, FranceGoogle Scholar
  7. 7.
    Samraj J, Menon MR, Christudas SP, Satyarajan PK (1966) Kokkan, a new disease of banana (Musa paradisiaca Linn.). Agric Res J Kerala 4:116Google Scholar
  8. 8.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  9. 9.
    Selvarajan R, Balasubramanian V (2008) Banana viruses. In: Rao GP (ed) Characterization diagnosis & management of plant viruses. Studium Press, Houston, pp 109–124Google Scholar
  10. 10.
    Singh SJ, Selvarajan R, Singh HP (2000) Detection of bract mosaic virus (kokkan disease) by electron microscopy and serology. In: Singh HP (eds) Banana-improvement, production and utilization. Proceedings of Conference in AIPUB, Tiruchirappali, India, pp 381–383Google Scholar
  11. 11.
    Shukla DD, Ward CR, Brunt AA (1994) The Potyviridae. CAB International, WallingfordGoogle Scholar
  12. 12.
    Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  13. 13.
    Thomas JE, Geering ADW, Gambley CF, Kessling AF, White M (1997) Purification, properties and diagnosis of banana bract mosaic potyvirus and its distinction from abaca mosaic potyvirus. Phytopathology 87:698–705PubMedCrossRefGoogle Scholar
  14. 14.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties, and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCrossRefGoogle Scholar
  15. 15.
    Urcuqui-Inchima S, Haenni AL, Bernardi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Molecular Virology Lab, Division of Crop ProtectionNational Research Centre for BananaTiruchirapalliIndia

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