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Archives of Virology

, Volume 157, Issue 2, pp 271–284 | Cite as

Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples

  • Stephen J. WylieEmail author
  • Hao Luo
  • Hua Li
  • Michael G. K. Jones
Original Article

Abstract

RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus, Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays.

Keywords

Coat Protein Bean Common Mosaic Virus Apple Chlorotic Leaf Spot Virus Onion Yellow Dwarf Virus Leek Yellow Stripe Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was funded by an Australian Research Council Linkage Grant (LP110200180) and the Murdoch University Institutes of Sustainable Ecosystems, and Crop and Plant Research. Thanks to Professor Kingsley Dixon and Mr. Steve Easton, Botanic Gardens and Parks Authority, for authorizing collections at Kings Park and for assistance in collecting and identifying the plants used from there.

Supplementary material

705_2011_1166_MOESM1_ESM.doc (108 kb)
Supplementary material 1 (DOC 108 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Stephen J. Wylie
    • 1
    Email author
  • Hao Luo
    • 1
  • Hua Li
    • 1
  • Michael G. K. Jones
    • 1
  1. 1.Plant Virology Group, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and BiotechnologyMurdoch UniversityPerthAustralia

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