Archives of Virology

, Volume 156, Issue 12, pp 2215–2220 | Cite as

Complete genome sequences and phylogenetic analysis of hepatitis delta viruses isolated from nine Turkish patients

  • İnci Çelik
  • Ersin Karataylı
  • Emrah Çevik
  • S. Gökçe Kabakçı
  • Senem Ceren Karataylı
  • Bedia Dinç
  • Kubilay Çınar
  • Kendal Yalçın
  • Ramazan İdilman
  • Cihan Yurdaydın
  • A. Mithat Bozdayi
Original Article

Abstract

Hepatitis delta virus (HDV) is a subviral agent of hepatitis B virus (HBV), and its life cycle is dependent on HBV. It is commonly accepted that HDV has eight distinct genotypes. In this study, the complete nucleotide sequences of HDV genomes isolated from nine Turkish patients were obtained by RT-PCR using two pairs of primers that cover the entire HDV genome. PCR products were sequenced directly. The results showed that these 9 isolates were approximately 1680 base pairs in length and clustered in the genotype HDV-1 branch when phylogenetic analysis was done with the sequences together with the complete sequences of HDV genomes representing each genotype retrieved from GenBank. Analysis of a portion of the large hepatitis D antigen (L-HDAg) gene showed that sequence similarity among these Turkish isolates is between 87.4 and 97.1%, and the Turkish isolates have the most sequence similarity to HDV-1 (90.5%), while they have the least sequence similarity to HDV-3 (64.1%). Full-genome analysis indicates that the sequence similarity is between 80.7 and 95.4%, and the highest sequence similarity is 84.8% (between the Turkish isolates and HDV-1). The lowest sequence similarity is 56.4% (between the Turkish isolates and HDV-3). In conclusion, phylogenetic analysis shows that the Turkish HDV isolates belong to HDV-1.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • İnci Çelik
    • 1
  • Ersin Karataylı
    • 1
  • Emrah Çevik
    • 1
  • S. Gökçe Kabakçı
    • 1
  • Senem Ceren Karataylı
    • 1
  • Bedia Dinç
    • 2
  • Kubilay Çınar
    • 3
  • Kendal Yalçın
    • 4
  • Ramazan İdilman
    • 3
  • Cihan Yurdaydın
    • 1
    • 3
  • A. Mithat Bozdayi
    • 1
    • 3
  1. 1.Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara UniversityAnkaraTurkey
  2. 2.Microbiology DepartmentYüksek İhtisas HospitalAnkaraTurkey
  3. 3.Gastroenterology Department, School of MedicineAnkara UniversityAnkaraTurkey
  4. 4.Gastroenterology Department, School of MedicineDicle UniversityDiyarbakirTurkey

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