Abstract
Yellow mosaic disease was observed on Jatropha gossypifolia plants growing in Kathaupahadi, Madhya Pradesh, India, and whiteflies (Bemisia tabaci) were found in the vicinity. Association of a new begomovirus with yellow mosaic disease of J. gossypifolia has been detected by PCR using begomovirus DNA-A-specific primers. The complete DNA-A genome (~2.7 kb) of this virus isolate was amplified by rolling-circle amplification (RCA) followed by digestion with Bam HI. The ~2.7-kb amplicons was cloned and sequenced, and the data obtained were submitted to GenBank under accession numbers FJ177030. The genome of the virus isolate consisted of six open reading frames (ORFs): V2 (pre-coat protein) and V1 (coat protein) in the virion sense and C3 (REn protein), C2 (TrAP protein), C1 (replication-associated protein) and C4 (C4 protein) in the complementary sense. BLASTn analysis of the nucleotide sequence (2757 nt) of the viral genome (FJ177030) showed 84–85% identity and a distinct phylogenetic relationship with DNA-A of tomato leaf curl virus-Bangalore II (U38239) and tomato leaf curl Karnataka virus (AY754812). Based on its 85% sequence identity to all other begomoviruses known to date and ICTV species demarcating criteria (< 88% identity), the name Jatropha yellow mosaic India virus (JYMIV) is proposed. JYMIV is considered to be monopartite, as neither DNA-B nor DNA-β components associated with begomoviruses were detected.
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References
Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann App Biol 142:145–146
Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM and Harrison BD (2005) In: Virus taxonomy: VIII Report of the international committee on taxonomy of viruses, Elsevier Academic Press, London 301–326
Rybicki EP (1994) A phylogenetic and evolutionary justification for 3 genera of Geminiviridae. Arch Virol 139:49–77
Padidam M, Sawyer S, Fauquet CM (1999) Possible emergence of new geminiviruses by frequent recombination. Virology 265:218–225
Paximadis M, Idris AM, Torres-Jerez I, Villarreal A, Rey MEC, Brown JK (1999) Characterization of tobacco geminiviruses in the Old and New World. Arch Virol 144:703–717
Briddon RW, Stanley J (2006) Subviral agents associated with plant single-stranded DNA viruses. Virology 344:198–210
Roye M, Collins S, Maxwell DP (2006) First report of a begomovirus associated with the common weed Jatropha gossypifolia in Jamaica. Plant Pathol 55:286
Okoth VAG (1991) Whitefly as a vector of African cassava mosaic virus (ACMV). Namulonge research station, Ministry of Agriculture, Animal Industry and Fisheries, Kampala. Plant Dis 77:340–347
Aswatha Narayana DS, Rangaswamy KT, Shankarappa KS, Maruthi MN, Reddy Laksminarayana CN, Rekha AR, Murthy Keahava KV (2007) District begomoviruses closely related to cassava mosaic viruses cause Indian Jatropha mosaic disease. Int J Virol 3:1–11
Raj SK, Snehi SK, Kumar S, Khan MS, Pathre U (2008) First molecular identification of a begomovirus in India that is closely related to Cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australas Plant Dis Notes 3:69–71
Gao S, Qu J, Chua NH, Ye J (2010) A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas. Arch Virol 155:607–612
Singh R, Raj SK, Prasad V (2008) Molecular identification of a new strain of Squash leaf curl china virus infecting Cucurbita maxima in India. J Phytopathol 156:222–228
Dellaporta SL, Wood JJ, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Rojas MR, Gilbertson RL, Russel DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347
Padidam M, Beachy RN, Fauquet CM (1995) Classification and identification of geminiviruses using sequence comparisons. J Gen Virol 76:249–263
Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan P, Rishi N, Siwatch SS, Abdel-Salam AM, Brown JK, Zafar Y, Markham PG (2003) Diversity of DNAβ, a satellite molecule associated with some monopartite begomoviruses. Virology 312:106–121
Haible D, Kober S, Jeske H (2006) Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J Virol methods 135:9–16
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821
Pandey P, Mukhopadhya S, Naqvi AR, Mukherjee SK, Shekhawat GS, Choudhury NR (2010) Molecular characterization of two distinct monopartite begomoviruses infecting tomato in India. Virol J 7:337–346
Van Brunschot SL, Persley DM, Geering ADW, Campbell PR, Thomas JE (2010) Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules. Australas Plant Pathol 39:412–423
Zhang H, Xin-ying MA, Ya-juan Q, Xue-ping Z (2010) Molecular characterization and infectivity of papaya leaf curl China virus infecting tomato in China. Biomed Biotechnol 11:109–114
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The authors are thankful to the Director of the CSIR-National Botanical Research Institute, Lucknow, U. P., India, for use of the facilities, and the Department of Biotechnology, New Delhi, India, for funding.
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Snehi, S.K., Raj, S.K., Khan, M.S. et al. Molecular identification of a new begomovirus associated with yellow mosaic disease of Jatropha gossypifolia in India. Arch Virol 156, 2303–2307 (2011). https://doi.org/10.1007/s00705-011-1118-5
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DOI: https://doi.org/10.1007/s00705-011-1118-5