Archives of Virology

, 156:1897 | Cite as

Genomic characterization of pea enation mosaic virus-2 from the Pacific Northwestern USA

  • B. Vemulapati
  • K. L. Druffel
  • S. D. Eigenbrode
  • A. Karasev
  • H. R. PappuEmail author
Annotated Sequence Record


Pea enation mosaic virus (PEMV) infects several legume crops, including chickpea (Cicer arietinum), faba bean (Vicia faba), lentil (Lens culinaris) and pea (Pisum sativum). The virus caused yield losses of food legumes in the Pacific Northwestern US during 1983, 1987 and 1990 [1]. Our recent surveys of pea and alfalfa fields in the states of Washington and Idaho, USA, have shown the prevalence of PEMV on pea. PEMV consists of a large (RNA-1 or PEMV-1) and a small (RNA-2 or PEMV-2) single-stranded positive-sense RNA, which are encapsidated separately into distinct isometric particles [2].

PEMV-2 is one of the seven distinct virus species in the genus Umbravirus. The other species of this genus are Carrot mottle virus (CMoV), Carrot mottle mimic virus (CMoMV), Groundnut rosette virus (GRV), Lettuce speckles mottle virus (LSMV), Tobacco mottle virus (TMoV) and Tobacco bushy top virus (TBTV) [3]. Members of this group lack the coat protein (CP) gene in their genomes and...


Deduce Amino Acid Sequence Faba Bean Recombination Detection Program Benton County Deduce Amino Acid Sequence Identity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported in part by funding from the USA Dry Peas and Lentil Council and a grant (no. 2008-511010-4522) from USDA NIFA RAMP. PPNS no. 0573, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Project no. WNP0 0545, Washington State University, Pullman, WA 99164-6430, USA.


  1. 1.
    Larsen RC (2001) Pea enation mosaic virus. In: Kraft JM, Pfleger FL (eds) Compendium of pea diseases and pests, American Phytopathological Society Press, pp 67Google Scholar
  2. 2.
    Demler SA, Borkhsenious ON, Rucker DG, de Zoeten GA (1994) Assessment of autonomy of replicative and structural functions encoded by the luteo-phase of pea enation mosaic virus. J Gen Virol 75:997–1007Google Scholar
  3. 3.
    Taliansky ME, Robinson DJ (2003) Molecular biology of umbraviruses: Phantom warriors. J Gen Virol 84:1951–1960PubMedCrossRefGoogle Scholar
  4. 4.
    Demler SA, Rucker DG, de Zoeten GA (1993) The chimeric nature of the genome of Pea enation mosaic virus: the independent replication of RNA-2. J Gen Virol 74:1–14PubMedCrossRefGoogle Scholar
  5. 5.
    Demler SA, de Zoeten GA (1991) The nucleotide sequence and luteovirus-like nature of RNA-1 of an aphid non-transmissible strain of Pea enation mosaic virus. J Gen Virol 72:1819–1834Google Scholar
  6. 6.
    Vemulapati B, Druffel KL, Eigenbrode SD, Karasev A, Pappu HR (2010) Molecular characterization of Pea enation mosaic virus and Bean leafroll virus from the Pacific Northwest, USA. Arch Virol 155:1713–1715PubMedCrossRefGoogle Scholar
  7. 7.
    Ryabov EV, Fraser G, Mayo MA, Taliansky M (2001) Umbravirus gene expression helps Potato leafroll virus to invade mesophyll tissues and to be transmitted mechanically between plants. Virology 286:363–372PubMedCrossRefGoogle Scholar
  8. 8.
    Ryabov EV, Robinson DJ, Taliansky M (2001) Umbravirus-encoded proteins both stabilize heterologous viral RNA and mediate its systemic movement in some plant species. Virology 288:391–400PubMedCrossRefGoogle Scholar
  9. 9.
    Reisman D, de Zoeten GA (1982) A covalently linked protein at the 5′-ends of the genomic RNAs of Pea enation mosaic virus. J Gen Virol 62:187–190CrossRefGoogle Scholar
  10. 10.
    Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  11. 11.
    Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • B. Vemulapati
    • 1
  • K. L. Druffel
    • 1
  • S. D. Eigenbrode
    • 2
  • A. Karasev
    • 2
  • H. R. Pappu
    • 1
    Email author
  1. 1.Department of Plant PathologyWashington State UniversityPullmanUSA
  2. 2.Department of Plant, Soil and Entomological SciencesUniversity of IdahoMoscowUSA

Personalised recommendations