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Archives of Virology

, Volume 156, Issue 10, pp 1707–1715 | Cite as

Synergistic effects of Cydia pomonella granulovirus GP37 on the infectivity of nucleopolyhedroviruses and the lethality of Bacillus thuringiensis

  • Xiangyang Liu
  • Xiaohe Ma
  • Chengfeng Lei
  • Yuzhou Xiao
  • Zhongxin Zhang
  • Xiulian SunEmail author
Original Article

Abstract

To initiate an efficient primary infection, it is important for baculovirus virions to penetrate through the peritrophic membrane (PM) of the host insect. It is frequently reported that enhancins of baculoviruses significantly enhance viral infection by degrading the various protein components of PMs. However, not all baculoviruses encode enhancins. GP37s of baculoviruses share high amino acid identity with fusolins, synergistic factors found in entomopoxviruses. In this study, a truncated Cydia pomonella granulovirus GP37 was expressed in Escherichia coli. The expressed GP37 effectively bound to chitin, and binding occurred predominantly within 3 h. GP37 altered the protein profiles of Spodoptera exigua PMs, from which a 50-kDa protein was dissociated. Droplet-feeding bioassays indicated that GP37 significantly enhanced the infectivity of nucleopolyhedroviruses (NPVs) and the lethality of Bacillus thuringiensis (Bt) in S. exigua larvae. This is the first demonstration of the enhancement of NPVs and Bt infection by a baculovirus GP37.

Keywords

Chitin Potency Ratio Peritrophic Membrane Midgut Epithelial Cell Chitin Powder 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We appreciate the support from the National Natural Science Foundation of China (30170085), the 973 project (2009CB118903) and the Knowledge Innovation Programs of the Chinese Academy of Sciences (KSCX2-EW-G-16).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xiangyang Liu
    • 1
    • 2
  • Xiaohe Ma
    • 1
    • 2
  • Chengfeng Lei
    • 1
  • Yuzhou Xiao
    • 1
  • Zhongxin Zhang
    • 1
  • Xiulian Sun
    • 1
    Email author
  1. 1.Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina

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