A novel gammaherpesvirus isolated from a black-tailed prairie dog (Cynomys ludovicianus)
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A new gammaherpesvirus, tentatively named cynomys herpesvirus 1 (CynGHV-1), was isolated from a black-tailed prairie dog (Cynomys ludovicianus). CynGHV-1 replicated cytopathogenically to moderate titers in various cell lines. Ten kb of the CynGHV-1 genome was sequenced using degenerate PCR and genomic cloning. Sequence similarities were found to different genes from known gammaherpesviruses. Phylogenetic analysis suggested that CynGHV-1 was in fact a novel virus closely related to representatives of different genera and unclassified members of the subfamily Gammaherpesvirinae. However, CynGHV-1 could not be assigned to any particular genus and therefore remains unclassified.
KeywordsGammaherpesvirus Prairie dog Novel virus
We would like to thank Mrs. Carol E. Hearne for the electron microscopy data. We are also very grateful to Dr. Geoff J. Letchworth for his advice and enthusiasm throughout the duration of the project as well as critical review of this manuscript. The authors also wish to thank Dr. Charles H. Calisher and Dr. J. Jeffrey Root, (CSU, Fort Collins) for providing the prairie dog serum samples. Bioinformatic analysis by CT-R was supported by NIH Grant P20 RR016474 from the INBRE Program of the National Center for Research Resources.
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