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Archives of Virology

, Volume 156, Issue 10, pp 1835–1840 | Cite as

A novel gammaherpesvirus isolated from a black-tailed prairie dog (Cynomys ludovicianus)

  • Brandy Nagamine
  • Leandro Jones
  • Christian Tellgren-Roth
  • Jacqueline Cavender
  • Ana C. Bratanich
Brief Report

Abstract

A new gammaherpesvirus, tentatively named cynomys herpesvirus 1 (CynGHV-1), was isolated from a black-tailed prairie dog (Cynomys ludovicianus). CynGHV-1 replicated cytopathogenically to moderate titers in various cell lines. Ten kb of the CynGHV-1 genome was sequenced using degenerate PCR and genomic cloning. Sequence similarities were found to different genes from known gammaherpesviruses. Phylogenetic analysis suggested that CynGHV-1 was in fact a novel virus closely related to representatives of different genera and unclassified members of the subfamily Gammaherpesvirinae. However, CynGHV-1 could not be assigned to any particular genus and therefore remains unclassified.

Keywords

Gammaherpesvirus Prairie dog Novel virus 

Notes

Acknowledgments

We would like to thank Mrs. Carol E. Hearne for the electron microscopy data. We are also very grateful to Dr. Geoff J. Letchworth for his advice and enthusiasm throughout the duration of the project as well as critical review of this manuscript. The authors also wish to thank Dr. Charles H. Calisher and Dr. J. Jeffrey Root, (CSU, Fort Collins) for providing the prairie dog serum samples. Bioinformatic analysis by CT-R was supported by NIH Grant P20 RR016474 from the INBRE Program of the National Center for Research Resources.

Supplementary material

705_2011_1024_MOESM1_ESM.pdf (1.3 mb)
Figure S1. Panoramic view alignment of complete pol aminoacid sequences of CynGHV-1 and similar sequences from GenBank obtained by Blast analysis. Alignment positions for which data was available were given a colored background. Sequence positions corresponding to gaps were given a black background (and thus are visualized as black boxes). Sequence positions that are missing (because of disparities in the length of sequences) are indicated with question marks (?) and have no background. (PDF 1333 kb)
705_2011_1024_MOESM2_ESM.tif (13.8 mb)
Figure S2. Parsimony tree of complete pol amino acid sequences of CynGHV-1 and similar sequences from GenBank as shown in Fig S1. Numbers on tree nodes indicate bootstrap (n=100) support. Branches with bootstrap supports lower than 50 were collapsed. Maximum Likelihood and Bayesian analyses (not shown) gave similar results. Branch lengths are proportional to the number of amino acid substitutions (bar = 10 substitutions). (TIFF 14080 kb)
705_2011_1024_MOESM3_ESM.doc (208 kb)
Supplementary material 3 (DOC 208 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Brandy Nagamine
    • 1
  • Leandro Jones
    • 4
  • Christian Tellgren-Roth
    • 2
    • 3
  • Jacqueline Cavender
    • 1
  • Ana C. Bratanich
    • 1
    • 5
  1. 1.Department of Veterinary ScienceUniversity of WyomingLaramieUSA
  2. 2.Center for Rural Health Research and EducationUniversity of WyomingLaramieUSA
  3. 3.Rudbecklaboratoriet, Department of Genetics and PathologyUppsala UniversityUppsalaSweden
  4. 4.Division of Molecular BiologyEstación de Fotobiología Playa Unión, CC 15Playa UniónArgentina
  5. 5.Cátedra de Virología, Facultad de Ciencias VeterinariasUniversidad de Buenos AiresBuenos AiresArgentina

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