Complete nucleotide sequence of a begomovirus and associated betasatellite infecting croton (Croton bonplandianus) in Pakistan
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The complete sequences of a begomovirus and an associated betasatellite isolated from Croton bonplandianus originating from Pakistan were determined. The sequence of the begomovirus showed the highest level of nucleotide sequence identity (88.9%) to an isolate of papaya leaf curl virus and thus represents a new species, for which we propose the name Croton yellow vein virus (CYVV). The sequence of the betasatellite showed the highest levels of sequence identity (82 to 98.4%) to six sequences in the databases that have yet to be reported, followed by isolates of tomato leaf curl Joydebpur betasatellite (48.7 to 52.5%). This indicates that the betasatellite identified here (and the six sequences in the databases) is an isolate of a newly identified species for which the name Croton yellow vein mosaic betasatellite (CroYVMB) is proposed. For the begomovirus, an analysis of the sequence indicates that it has a recombinant origin.
KeywordsCroton Nucleotide Sequence Identity Yellow Vein Minor Parent Recombinant Fragment
K.H. was supported by a Ph.D. fellowship from the Higher Education Commission (HEC), Government of Pakistan. R.W.B. is supported by the HEC under the “Foreign Faculty Hiring Program”. This research was supported by a research grant under a “Pakistan-USA Collaborative Research” project between NIBGE and Danforth Plant Science Center, USA.
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