Archives of Virology

, Volume 156, Issue 8, pp 1345–1358

A nationwide survey of hepatitis E virus (HEV) infection in wild boars in Japan: identification of boar HEV strains of genotypes 3 and 4 and unrecognized genotypes

  • Yukihiro Sato
  • Hiroyuki Sato
  • Keisuke Naka
  • Satoshi Furuya
  • Haruhisa Tsukiji
  • Koji Kitagawa
  • Yoshihide Sonoda
  • Takanobu Usui
  • Hirotsugu Sakamoto
  • Sumi Yoshino
  • Yuko Shimizu
  • Masaharu Takahashi
  • Shigeo Nagashima
  • Jirintai
  • Tsutomu Nishizawa
  • Hiroaki Okamoto
Original Article

DOI: 10.1007/s00705-011-0988-x

Cite this article as:
Sato, Y., Sato, H., Naka, K. et al. Arch Virol (2011) 156: 1345. doi:10.1007/s00705-011-0988-x

Abstract

To investigate the nationwide prevalence of hepatitis E virus (HEV) infection and to characterize HEV genomes among Japanese wild boars (Sus scrofa leucomystax), 578 boars captured in 25 prefectures from 2003 to 2010 were studied. Anti-HEV IgG was detected in 8.1%, and HEV RNA in 3.3% of boars. Among the 19 boar HEV isolates obtained from infected boars, 14 isolates (74%) were classified as genotype 3, 4 isolates (21%) as genotype 4, and the remaining isolate (wbJOY_06) was distantly related to all known HEV isolates of genotypes 1-4, differing by 18.4-25.0% and 18.0-24.3% within the 412-nucleotide sequence of ORF1 and ORF2, respectively. A genotype 4 boar HEV isolate (wbJGF_08-1) obtained herein shared 98.6% identity over the entire genome with a human HEV isolate obtained from a patient who developed acute hepatitis after consuming undercooked wild boar meat, suggesting that wild boars are also reservoirs for genotype 4 HEV in humans.

Supplementary material

705_2011_988_MOESM1_ESM.eps (1.3 mb)
Supplementary material 1 (EPS 1341 kb)
705_2011_988_MOESM2_ESM.xls (26 kb)
Supplementary material 2 (XLS 25 kb)
705_2011_988_MOESM3_ESM.doc (70 kb)
Supplementary material 3 (DOC 70 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yukihiro Sato
    • 1
  • Hiroyuki Sato
    • 2
    • 3
  • Keisuke Naka
    • 4
    • 5
  • Satoshi Furuya
    • 6
    • 7
  • Haruhisa Tsukiji
    • 8
  • Koji Kitagawa
    • 9
    • 10
  • Yoshihide Sonoda
    • 11
  • Takanobu Usui
    • 12
    • 13
  • Hirotsugu Sakamoto
    • 14
    • 3
  • Sumi Yoshino
    • 15
    • 16
  • Yuko Shimizu
    • 17
  • Masaharu Takahashi
    • 18
  • Shigeo Nagashima
    • 18
  • Jirintai
    • 18
  • Tsutomu Nishizawa
    • 18
  • Hiroaki Okamoto
    • 18
  1. 1.Kamiichi General HospitalKamiichiJapan
  2. 2.Maniwa Kokuho Yubara Onsen HospitalManiwaJapan
  3. 3.Division of Gastroenterology, Department of Internal MedicineJichi Medical University School of MedicineShimotsukeJapan
  4. 4.Tessei ClinicNiimiJapan
  5. 5.Okamura Isshindo HospitalOkayamaJapan
  6. 6.Koshu City Kokuho Enzan ClinicKoshuJapan
  7. 7.Yamanashi City Makioka HospitalYamanashiJapan
  8. 8.Ito Shimin HospitalItoJapan
  9. 9.Nishiazai-Machi Kokuho Nagahara ClinicNagahamaJapan
  10. 10.Koka City Shigaraki Chuo HospitalKokaJapan
  11. 11.Nara City Tawara ClinicNaraJapan
  12. 12.Hitachiomiya City Kokuho Miwa ClinicHitachiomiyaJapan
  13. 13.Tokai Village HospitalNakaJapan
  14. 14.Kitaibaraki City General HospitalKitaibarakiJapan
  15. 15.Takinomiya General HospitalAyagawaJapan
  16. 16.Kagawa Rosai HospitalMarugameJapan
  17. 17.Tosei General HospitalSetoJapan
  18. 18.Division of Virology, Department of Infection and ImmunityJichi Medical University School of MedicineShimotsukeJapan

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