Archives of Virology

, 156:1305 | Cite as

DNA genome of spontaneously occurring deletion mutants of herpes simplex virus type 1 lacking one copy of the inverted repeat sequences of the L component

Original Article


Three non-engineered, spontaneously occurring herpes simplex virus type 1 (HSV-1) mutants (GN52, GN82, and GN91) that have a deletion of approximately 10 kbp (including a part of the UL55 gene, the entire UL56 gene, and one copy of the inverted repeat sequences of the L component (RL)) and retain the a sequence were isolated. The yields of the mutants at 24 h post-adsorption in cultured cells were comparable to that of an HSV-1 isolate (GN28) without the deletion. Although the three mutants lost one copy of RL, the L component in replicative intermediates of the mutants inverted. DNA replicative intermediates of the three mutants were flanked by the L component, like those of GN28. The three mutants were generated through recombination involving regions around the authentic cleavage site in the a sequence, suggesting an important role of the a sequence in the diversification of herpesviruses.



Part of this study was supported by grants from the Ministry of Education, Science, Technology, Sports and Culture of Japan.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Nutrition and Health Science, Faculty of Human Environmental ScienceFukuoka Woman’s UniversityFukuokaJapan
  2. 2.Division of Human Molecular Genetics, Center for Genetic Information, Medical Institute of BioregulationKyushu UniversityFukuokaJapan

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