Serotype-specific and cross-reactive neutralizing antibody responses in cynomolgus monkeys after infection with multiple dengue virus serotypes
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Neutralizing antibody responses were examined in monkeys after dengue virus infections. In monkeys that had been infected once or twice with DENV-2, neutralizing antibody was cross-reactive with all four serotypes after secondary or tertiary infection with DENV-3. In monkeys that had been inoculated with DENV-1 and DENV-2 in the primary and secondary infections, neutralizing antibody titers did not increase after tertiary infection with DENV-3. These results indicate that antibody responses after secondary and tertiary infections with different serotypes are cross-reactive with all four serotypes, consistent with what has been observed in humans, and suggest that monkeys are useful for determining neutralizing antibody responses.
KeywordsDengue virus Monkey Neutralyzing antibody Serotype cross-reactivity
Animal procedures were approved by the Committees on Biosafety and Animal Handling and Ethical Regulations of the National Institute of Infectious, Diseases, Japan. This work was supported by the Research on Emerging and Re-emerging Infectious Diseases from Ministry of Health, Labour and Welfare, Japan (H17-Shinkou-019 and H20-Shinkou-ippan-015), and the Research on Biological Resources and Animal Models for Drug Development from Ministry of Health, Labour and Welfare, Japan (H19-Seibutsushigen-ippan-003).
- 2.Gubler DJ, Kuno G, Markoff L (2009) Flaviviruses. In: Knipe DM, Howley PM (eds) Fields Virology. Lippincott Williams & Wilkins, New York, pp 1155–1251Google Scholar
- 5.Reed LJ, Muench HA (1938) Simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497Google Scholar
- 6.Innis BL (1997) Antibody responses to dengue virus infection. In: Gubler DJ, Kuno G (eds) Dengue and dengue hemorrhagic fever. Cab International, New York, pp 221–243Google Scholar
- 8.Sa-ngasang A, Anantapreecha S, A-nuegoonpipat A, Chanama S, Wibulwattanakij S, Pattanakul K, Sawanpanyalert P, Kurane I (2006) Specific IgM and IgG responses in primary and secondary dengue virus infections determined by enzyme-linked immunosorbent assay. Epidemiol Infect 134:820–825PubMedCrossRefGoogle Scholar
- 10.Endy TP, Nisalak A, Chunsuttitwat S, Vaughn DW, Green S, Ennis EA, Rothman A, Libraty D (2004) Relationship of preexisting dengue virus (DV) neutralizing antibody levels to viremia and severity of disease in a prospective cohort of DV infection in Thailand. J infect Dis 189:990–1000PubMedCrossRefGoogle Scholar