Molecular characterization of a new begomovirus and divergent alphasatellite from tomato in Cameroon
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Tomato (Solanum lycopersicum) is a crop of growing importance throughout West and Central Africa. Recently, several begomoviruses (genus Begomovirus; family Geminiviridae) have been associated with leaf curl and yellow leaf curl symptoms in tomato crops in West Africa, including tomato yellow leaf curl Mali virus (TYLCMLV), tomato yellow crumple virus (TYLCrV), tomato leaf curl Mali virus (ToLCMLV), tomato leaf curl Nigeria virus (ToLCNGV), tomato leaf curl Ghana virus (ToLCGHV), tomato leaf curl Kumasi virus (ToLCKuV) and tomato leaf curl Togo virus (ToLCTGV) [3, 7, 10, 15]. These viruses are transmitted by the whitefly Bemisia tabaci (Genn.) sibling species group, and they have a genome of single-stranded (ss) DNA (about 2.7-2.8 kb) with an organization typical for Old World monopartite begomoviruses . In addition, many monopartite begomoviruses are associated with alphasatellite and betasatellite molecules that may influence symptom development, pathogenicity, and virulence [1, 3...
KeywordsYellow Leaf Yellow Vein Tomato Leaf Tomato Sample Asymptomatic Plant
This research was jointly supported by several foundations and organizations to which we express our sincere gratitude: International Foundation of Science, Stockholm, Sweden, through a grant to W.N. Leke; Rothamsted International, through the Rothamsted International African Fellows programme; The Swedish Institute; USAID, through the IPM-CRSP Insect-Transmitted Virus Global Themes project; The Royal Physiographic Society in Lund, Sweden. W.N. Leke completed this study as part of his Ph.D. studies. We sincerely thank the anonymous referees for their useful comments and suggestions.
- 3.Chen LF, Rojas MR, Kon T, Gamby K, Xoconostle-Cazares B, Gilbertson RL (2009) A severe symptom phenotype in tomato in Mali is caused by a reassortant between a novel recombinant begomovirus (Tomato yellow leaf curl Mali virus) and a betasatellite. Mol Plant Pathol 10:415–430PubMedCrossRefGoogle Scholar
- 6.Leke WN, Ramsell JNE, Njualem DK, Titanji VPK, Legg JP, Fondong VN, Brown JK, Kvarnheden A (2007) FTA technology facilitates detection and identification of begomoviruses from okra plants in Cameroon. Afr Crop Sci Soc Conf Proc 8:655–660Google Scholar
- 10.Osei MK, Akromah R, Shih SL, Lee LM, Green SK (2008) First report and molecular characterization of three distinct begomoviruses associated with tomato leaf curl disease in Ghana. Plant Dis 92:1585–1585Google Scholar
- 15.Zhou YC, Noussourou M, Kon T, Rojas MR, Jiang H, Chen L-F, Gamby K, Foster R, Gilbertson RL (2008) Evidence of local evolution of tomato-infecting begomovirus species in West Africa: characterization of tomato leaf curl Mali virus and tomato leaf crumple virus from Mali. Arch Virol 153:693–706PubMedCrossRefGoogle Scholar