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Archives of Virology

, Volume 156, Issue 5, pp 911–916 | Cite as

The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus

  • Akihiro Hiraguri
  • Osamu Netsu
  • Takumi Shimizu
  • Tamaki Uehara-Ichiki
  • Toshihiro Omura
  • Nobumitsu Sasaki
  • Hiroshi Nyunoya
  • Takahide Sasaya
Brief Report

Abstract

The nonstructural protein pC6 encoded by rice grassy stunt virus is thought to correspond functionally to the nonstructural protein pC4 of rice stripe virus, which can support viral cell-to-cell movement. In a trans-complementation experiment with a movement-defective tomato mosaic virus, pC6 and pC4 facilitated intercellular transport of the virus. Transient expression of pC6, fused with green fluorescent protein, in epidermal cells was predominantly observed close to the cell wall as well as in a few punctate structures, presumably associated with plasmodesmata. These results suggest that pC6 has a role similar to that of pC4 in viral cell-to-cell movement.

Keywords

Tobacco Mosaic Virus Nonstructural Protein Rice Stripe Virus Microprojectile Bombardment Punctate Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to Dr. A. Tamai (Ishikawa Prefectural University, Ishikawa, Japan) and Dr. T. Meshi (National Institute of Agrobiological Sciences, Tsukuba, Japan) for providing piL.erG3. This study was supported by the Program for Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN) of Japan and a Grant-in-Aid for Young Scientists (B) (to N.S., no. 20780030) from the Ministry of Education, Culture, Sports, and Science.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Akihiro Hiraguri
    • 1
    • 2
  • Osamu Netsu
    • 1
    • 2
  • Takumi Shimizu
    • 1
    • 2
  • Tamaki Uehara-Ichiki
    • 1
    • 2
  • Toshihiro Omura
    • 1
    • 2
  • Nobumitsu Sasaki
    • 1
    • 2
  • Hiroshi Nyunoya
    • 1
    • 2
  • Takahide Sasaya
    • 1
    • 2
  1. 1.National Agricultural Research CenterTsukubaJapan
  2. 2.Cooperative Educational Study and Research Facilities, Gene Research Center, Tokyo University of Agriculture and TechnologyFuchuJapan

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