The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus
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The nonstructural protein pC6 encoded by rice grassy stunt virus is thought to correspond functionally to the nonstructural protein pC4 of rice stripe virus, which can support viral cell-to-cell movement. In a trans-complementation experiment with a movement-defective tomato mosaic virus, pC6 and pC4 facilitated intercellular transport of the virus. Transient expression of pC6, fused with green fluorescent protein, in epidermal cells was predominantly observed close to the cell wall as well as in a few punctate structures, presumably associated with plasmodesmata. These results suggest that pC6 has a role similar to that of pC4 in viral cell-to-cell movement.
KeywordsTobacco Mosaic Virus Nonstructural Protein Rice Stripe Virus Microprojectile Bombardment Punctate Structure
The authors are grateful to Dr. A. Tamai (Ishikawa Prefectural University, Ishikawa, Japan) and Dr. T. Meshi (National Institute of Agrobiological Sciences, Tsukuba, Japan) for providing piL.erG3. This study was supported by the Program for Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN) of Japan and a Grant-in-Aid for Young Scientists (B) (to N.S., no. 20780030) from the Ministry of Education, Culture, Sports, and Science.
- 4.Laporte C, Vetter G, Loudes AM, Robinson DG, Hillmer S, Stussi-Garaud C, Ritzenthaler C (2003) Involvement of the secretory pathway and the cytoskeleton in intracellular targeting and tubule assembly of Grapevine fanleaf virus movement protein in tobacco BY-2 cells. Plant Cell 15:2058–2075PubMedCrossRefGoogle Scholar
- 9.Mitsuhara I, Ugaki M, Hirochika H, Ohshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, Ueno K, Mochizuki A, Tanimoto H, Tsugawa H, Otsuki Y, Ohashi Y (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol 37:49–59PubMedGoogle Scholar