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Archives of Virology

, Volume 156, Issue 4, pp 685–688 | Cite as

Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

  • Rui Wu
  • Yongxiang Tian
  • Junhua Deng
  • Keli Yang
  • Wangwang Liang
  • Rui Guo
  • Zhengying Duan
  • Zewen Liu
  • Danna Zhou
  • Diping Xu
Brief Report

Abstract

In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.

Keywords

Japanese encephalitis virus Swine Mouse Adaptation Pathogenicity 

Notes

Acknowledgments

This study was supported by Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding (2010ZD150) and National Science & Technology Pillar Program (2006BAD06A18-6).

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Rui Wu
    • 1
    • 2
    • 3
  • Yongxiang Tian
    • 1
    • 2
  • Junhua Deng
    • 1
  • Keli Yang
    • 1
  • Wangwang Liang
    • 1
  • Rui Guo
    • 1
  • Zhengying Duan
    • 1
  • Zewen Liu
    • 1
  • Danna Zhou
    • 1
  • Diping Xu
    • 1
    • 2
  1. 1.Institute of Animal Husbandry and Veterinary ScienceHubei Academy of Agricultural SciencesWuhanChina
  2. 2.Hubei Key Laboratory of Animal Embryo Engineering and Molecular BreedingWuhanChina
  3. 3.National Engineering Research Center for Animal DrugsLuoyang Pu-like Bio-engineering Co. LtdLuoyangChina

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