Archives of Virology

, Volume 156, Issue 2, pp 253–261 | Cite as

Genetic diversity of Newcastle disease viruses isolated from domestic poultry species in Eastern China during 2005–2008

  • Shuang Wu
  • Weiwei Wang
  • Chunfeng Yao
  • Xiaoquan Wang
  • Shunlin Hu
  • Junping Cao
  • Yantao Wu
  • Wenbo Liu
  • Xiufan LiuEmail author
Original Article


Seventy-nine Newcastle disease viruses (NDV) isolated from clinical specimens of different poultry species including chickens, pigeons (Columba livia), geese and ostriches in Eastern China during 2005–2008 were characterized biologically and phylogenetically. The results showed genetic diversity of these viruses: three class I viruses and one genotype I and 12 genotype II viruses of class II circulating in chickens were avirulent; four genotype VIb viruses isolated from pigeons were moderately virulent; and two genotype III viruses and 57 genotype VIId viruses were highly virulent. The three class I viruses were further classified as genotypes 2 and 3. The very high F protein sequence identity of one genotype I virus with strain Queensland V4 and 12 genotype II viruses with strain La Sota indicated that these viruses originated from the two vaccine strains. Two genotype III viruses shared greater than 99% sequence identity with the moderately virulent vaccine strain Mukteswar but exhibited significantly higher virulence, suggesting that they evolved from the vaccine virus and that the Mukteswar vaccine should be banned in China. Fifty-seven of the 63 virulent NDVs in this study belonged to genotype VIId, indicating its predominance in Eastern China. Genotype VIId viruses could be further classified into two subgroups. Four of the five NDVs isolated from pigeons belonged to genotype VIb, indicating its host-specific preference. Both the genotype VIb and VIId NDVs showed low amino acid similarity to the vaccine strains currently used in China, implying the urgent need to develop better vaccines against the most prevalent NDVs in China.


Newcastle Disease Virus Vaccine Strain Newcastle Disease Chicken Embryo Fibroblast Chicken Flock 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by National Natural Science Foundation of China (key program, grant number: 30630048) and National Key Technologies R&D Program of China (grant numbers: 2006BAD06A03; 2006BAD06A08; 2006BAD06A16).


  1. 1.
    Afonso CL (2008) Not so fast on recombination analysis of Newcastle disease virus J Virol 82: 9303Google Scholar
  2. 2.
    Alexander DJ (1989) Newcastle disease. In: Purchase HG, Arp LH, Domermuth CH, Pearson JE. (Eds.), A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 3rd ed. American Association of Avian Pathologists, Kenneth Square, PA, : 114-120Google Scholar
  3. 3.
    Alexander DJ, Campbell G, Manvell RJ, Collins MS, Parsons G, McNulty MS (1992) Characterisation of an antigenically unusual virus responsible for two outbreaks of Newcastle disease in the Republic of Ireland in 1990. Vet Rec 130: 65-68Google Scholar
  4. 4.
    Alexander DJ (1997) Newcastle disease and other Paramyxoviridae infections. In: Calnek BW, Barnes HJ, Beard CW, McDougald L, Saif YM, editors. Diseases of poultry Ames: Iowa State University Press: 541-569Google Scholar
  5. 5.
    Alexander DJ (2003) Newcastle disease, other avian paramyxoviruses, and pneumovirus infections. 11th ed. In "Diseases of Poultry" (Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR , Swayne DE, Eds.). Iowa State University Press, Ames: pp. 64-87.Google Scholar
  6. 6.
    Chambers P, Millar NS, Emmerson PT (1986) Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. J Gen Virol 67 ( Pt 12): 2685-2694Google Scholar
  7. 7.
    Cho SH, Kwon HJ, Kim TE, Kim JH, Yoo HS, Kim SJ (2008) Variation of a newcastle disease virus hemagglutinin-neuraminidase linear epitope. J Clin Microbiol 46: 1541-1544Google Scholar
  8. 8.
    Czegledi A, Ujvari D, Somogyi E, Wehmann E, Werner O, Lomniczi B (2006) Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications. Virus Res 120: 36-48Google Scholar
  9. 9.
    Dai Y, Liu M, Li W (2008) Protective efficacy of commercial Newcastle disease vaccines against challenge of goose origin virulent Newcastle disease virus in geese. Avian Dis 52: 467-471Google Scholar
  10. 10.
    de Leeuw O, Peeters B (1999) Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae. J Gen Virol 80: 131-136Google Scholar
  11. 11.
    Harper DR (1989) A novel plaque assay system for paramyxoviruses. J Virol Methods 25: 347-350Google Scholar
  12. 12.
    Hu S, Ma H, Wu Y, Liu W, Wang X, Liu Y, Liu X (2009) A vaccine candidate of attenuated genotype VII Newcastle disease virus generated by reverse genetics. Vaccine 27: 904-910Google Scholar
  13. 13.
    Huang Y, Wan HQ, Liu HQ, Wu YT, Liu XF (2004) Genomic sequence of an isolate of Newcastle disease virus isolated from an outbreak in geese: a novel six nucleotide insertion in the non-coding region of the nucleoprotein gene. Brief Report. Arch Virol 149: 1445-1457Google Scholar
  14. 14.
    Kattenbelt JA, Stevens MP, Gould AR (2006) Sequence variation in the Newcastle disease virus genome. Virus Res 116: 168-184Google Scholar
  15. 15.
    Kim L, King D, Curry P, Suarez D, Swayne D, Stallknecht D, Slemons R, Pedersen J, Senne D, Winker K (2007a) Phylogenetic diversity among low virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to poultry-origin isolates. J Virol: 12641-12653Google Scholar
  16. 16.
    Kim L, King D, Suarez D, Wong C, Afonso CL (2007b) Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR. J Clin Microbiol 45: 1310-1314Google Scholar
  17. 17.
    Lamb RA, Kolakofsky D (2002) Paramyxoviridae: the viruses and their replication. In: Fields BB, Kniepe DM, Howley PM (Eds) Fundamental Virology Philadelphia: Lippincott-Raven Publishers: 1305-1340Google Scholar
  18. 18.
    Liu H, Wang Z, Wu Y, Zheng D, Sun C, Bi D, Zuo Y, Xu T (2007) Molecular epidemiological analysis of Newcastle disease virus isolated in China in 2005. J Virol Methods 140: 206-211Google Scholar
  19. 19.
    Liu H, Wang Z, Wang Y, Sun C, Zheng D, Wu Y (2008) Characterization of Newcastle disease virus isolated from waterfowl in China. Avian Dis 52: 150-155Google Scholar
  20. 20.
    Liu H, Wang Z, Wu Y, Sun C, Zheng D, Xu T, Li J (2008) Molecular characterization and phylogenetic analysis of new Newcastle disease virus isolates from the mainland of China. Res Vet Sci 85: 612-616Google Scholar
  21. 21.
    Liu X, Wan H, Ni X, Wu Y, Liu W (2003) Pathotypical and genotypical characterization of strains of Newcastle disease virus isolated from outbreaks in chicken and goose flocks in some regions of China during 1985-2001. Arch Virol 148: 1387-1403Google Scholar
  22. 22.
    Liu X, Wang X, Wu S, Hu S, Peng Y, Xue F, Liu X (2009) Surveillance for avirulent Newcastle disease viruses in domestic ducks (Anas platyrhynchos and Cairina moschata) at live bird markets in Eastern China and characterization of the viruses isolated. Avian Pathol 38: 377-391Google Scholar
  23. 23.
    Lomniczi B, Wehmann E, Herczeg J, Ballagi-Pordany A, Kaleta E, Werner O, Meulemans G, Jorgensen P, Mante A, Gielkens A (1998) Newcastle disease outbreaks in recent years in western Europe were caused by an old (VI) and a novel genotype (VII). Archives of virology 143: 49-64Google Scholar
  24. 24.
    Mayo MA (2002a) A summary of taxonomic changes recently approved by ICTV. Arch Virol 147: 1655-1663Google Scholar
  25. 25.
    Mayo MA (2002b) Virus taxonomy - Houston 2002. Arch Virol 147 1071-1076Google Scholar
  26. 26.
    McGinnes LW, Morrison TG (1986) Nucleotide sequence of the gene encoding the Newcastle disease virus fusion protein and comparisons of paramyxovirus fusion protein sequences. Virus Res 5: 343-356Google Scholar
  27. 27.
    Miller P, King D, Afonso C, Suarez D (2007) Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge. Vaccine 25: 7238-7246Google Scholar
  28. 28.
    Neyt C, Geliebter J, Slaoui M, Morales D, Meulemans G, Burny A (1989) Mutations located on both F1 and F2 subunits of the Newcastle disease virus fusion protein confer resistance to neutralization with monoclonal antibodies. J Virol 63: 952-954Google Scholar
  29. 29.
    Panda A, Huang Z, Elankumaran S, Rockemann DD, Samal SK (2004) Role of fusion protein cleavage site in the virulence of Newcastle disease virus. Microb Pathog 36: 1-10Google Scholar
  30. 30.
    Panshin A, Shihmanter E, Weisman Y, rvell C, Lipkind M (2002) Antigenic heterogeneity among the field isolates of Newcastle disease virus (NDV) in relation to the vaccine strain:: 1. Studies on viruses isolated from wild birds in Israel. Comparative immunology, microbiology and infectious diseases 25: 95-108Google Scholar
  31. 31.
    Peeters B, de Leeuw O, Koch G, Gielkens A (1999) Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. J Virol 73: 5001-5009Google Scholar
  32. 32.
    Qin Z, Sun L, Ma B, Cui Z, Zhu Y, Kitamura Y, Liu W (2008) F gene recombination between genotype II and VII Newcastle disease virus. Virus research 131: 299-303Google Scholar
  33. 33.
    Qin ZM, Tan LT, Xu HY, Ma BC, Wang YL, Yuan XY, Liu WJ (2008) Pathotypical characterization and molecular epidemiology of Newcastle disease virus isolates from different hosts in China from 1996 to 2005. J Clin Microbiol 46: 601-611Google Scholar
  34. 34.
    Qiu X, Sun Q, Yao C, Dong L, Wu Y, Hu S, Liu X (2009) Full-length genome analysis of two genotype III velogenic Newcastle diseases virus strains reveals their close relationship with vaccine Mukteswar. Wei sheng wu xue bao= Acta Microbiologica Sinica (in Chinese) 49: 302Google Scholar
  35. 35.
    Rui Z, Juan P, Jingliang S, Jixun Z, Xiaoting W, Shouping Z, Xiaojiao L, Guozhong Z (2010) Phylogenetic characterization of Newcastle disease virus isolated in the mainland of China during 2001-2009. Vet Microbiol 141: 246-257Google Scholar
  36. 36.
    Seal B, Wise M, Pedersen J, Senne D, Alvarez R, Scott M, King D, Yu Q, Kapczynski D (2005) Genomic sequences of low-virulence avian paramyxovirus-1 (Newcastle disease virus) isolates obtained from live-bird markets in North America not related to commonly utilized commercial vaccine strains. Vet Microbiol 106: 7-16Google Scholar
  37. 37.
    Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596-1599Google Scholar
  38. 38.
    Toyoda T, Gotoh B, Sakaguchi T, Kida H, Nagai Y (1988) Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization. J Virol 62: 4427-4430Google Scholar
  39. 39.
    Ujvari D (2006) Complete nucleotide sequence of IT-227/82, an avian paramyxovirus type-1 strain of pigeons (Columba livia). Virus Genes 32: 49-57Google Scholar
  40. 40.
    Ujvari D, Wehmann E, Herczeg J, Lomniczi B (2006) Identification and subgrouping of pigeon type Newcastle disease virus strains by restriction enzyme cleavage site analysis. J Virol Methods 131: 115-121Google Scholar
  41. 41.
    Yu L, Wang Z, Jiang Y, Chang L, Kwang J (2001) Characterization of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan. J Clin Microbiol 39: 3512-3519Google Scholar
  42. 42.
    Yusoff K, Nesbit M, McCartney H, Meulemans G, Alexander DJ, Collins MS, Emmerson PT, Samson AC (1989) Location of neutralizing epitopes on the fusion protein of Newcastle disease virus strain Beaudette C. J Gen Virol 70 ( Pt 11): 3105-3109Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Shuang Wu
    • 1
  • Weiwei Wang
    • 1
  • Chunfeng Yao
    • 1
  • Xiaoquan Wang
    • 1
  • Shunlin Hu
    • 1
  • Junping Cao
    • 1
  • Yantao Wu
    • 1
  • Wenbo Liu
    • 1
  • Xiufan Liu
    • 1
    Email author
  1. 1.Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, School of Veterinary MedicineYangzhou UniversityYangzhouChina

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