Archives of Virology

, Volume 155, Issue 11, pp 1897–1899 | Cite as

The complete sequence of a UK strain of black raspberry necrosis virus

  • W. J. McGavin
  • L. S. McMenemy
  • S. A. MacFarlaneEmail author
Annotated Sequence Record


The complete nucleotide sequence of a UK strain of the sadwavirus Black raspberry necrosis virus (BRNV) was obtained by amplification and sequencing of virus RNA from infected plants grown in a raspberry plantation in Aylth, Scotland. The RNA1 was 7,572 nucleotides (nt) in size and RNA2 was 6,350 nt in size, each excluding the 3′ poly-A tail. The RNA1- and RNA2-encoded polyproteins are predicted to be processed into (RNA1) a protease cofactor, an RNA helicase, the VpG, a 3C-like protease, an RNA-dependent RNA polymerase and an AlkB protein, and (RNA2) a movement protein and two capsid proteins.


North American Black Raspberry Significant Sequence Diversity Raspberry Plant American Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was funded by the Scottish Government Rural and Environment Research and Analysis Directorate (RERAD). LMS was supported by a PhD scholarship jointly funded by SCRI and Sussex University.


  1. 1.
    Halgren A, Tzanetakis IE, Martin RR (2007) Identification, characterization, and detection of Black raspberry necrosis virus. Phytopathology 97:44–50CrossRefPubMedGoogle Scholar
  2. 2.
    Jones AT, Murant AF (1972) Some properties of a mechanically transmissible virus widespread in raspberry (Rubus idaeus) in Scotland. Plant Pathol 21:166–170CrossRefGoogle Scholar
  3. 3.
    Jones AT, Roberts IM (1977) Rubus host range, symptomatology and ultrastructural effects of a British isolate of black raspberry necrosis virus. Ann Appl Biol 86:381–387CrossRefGoogle Scholar
  4. 4.
    McGavin WJ, MacFarlane SA (2009) Rubus chlorotic mottle virus, a new sobemovirus infecting raspberry and bramble. Virus Res 139:10–13CrossRefPubMedGoogle Scholar
  5. 5.
    McGavin WJ, MacFarlane SA (2010) Sequence similarities between Raspberry leaf mottle virus, Raspberry leaf spot virus and the closterovirus Raspberry mottle virus. Ann Appl Biol 156:439–448CrossRefGoogle Scholar
  6. 6.
    Rott ME, Jelkmann W (2001) Characterization and detection of several filamentous viruses of cherry: adaptation of an alternative cloning method (DOP-PCR), and modification of an RNA extraction protocol. Eur J Plant Pathol 107:411–420CrossRefGoogle Scholar
  7. 7.
    Stace-Smith R (1955) Studies on Rubus virus diseases in British Columbia. II. Black raspberry necrosis. Can J Bot 33:314–322CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • W. J. McGavin
    • 1
  • L. S. McMenemy
    • 1
  • S. A. MacFarlane
    • 1
    Email author
  1. 1.Plant Pathology ProgrammeSCRI, InvergowrieDundeeUK

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