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Archives of Virology

, Volume 155, Issue 12, pp 1997–2006 | Cite as

Three genetic grapevine leafroll-associated virus 3 variants identified from South African vineyards show high variability in their 5′UTR

  • A. E. C. JoosteEmail author
  • H. J. Maree
  • D. U. Bellstedt
  • D. E. Goszczynski
  • G. Pietersen
  • J. T. Burger
Original Article

Abstract

Three genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) were identified in vineyards of the Western Cape, South Africa. The GLRaV-3 variants were identified by single-strand conformation polymorphism (SSCP) profiles generated from a region amplified in ORF5. ORF5 sequence data confirmed the three genetic variant groups, and a specific SSCP profile was assigned to each variant group. The results of SSCP analysis of this region in ORF5 showed that this method gives a fast and reliable indication of the GLRaV-3 variant status of a plant, which in many instances showed mixed infections. The full genome sequence of one representative of each variant group i.e. isolates 621 (group I), 623 (group II) and PL-20 (group III), was determined by sequencing overlapping cloned fragments of these isolates. The sequences of genomic 5′ ends of these isolates were determined by RLM-RACE. Sequence alignment of the 5′UTRs indicated significant sequence and length variation in this region between the three South African variant groups. Alignment of the Hsp70h and CP gene regions of these isolates with those of isolates from elsewhere in the world, followed by phylogenetic analysis, further supported the presence of three variants of GLRaV-3 in South Africa and the presence of two or three additional variant groups elsewhere in the world.

Keywords

Coat Protein Variant Group Coat Protein Gene Citrus Tristeza Virus Online Resource Table 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The research in this study was funded by the South African industry partner, Winetech, in collaboration with the National Research Foundation’s THRIP programme and ARC-PPRI. We acknowledge the assistance of staff at the ARC-PPRI and Stellenbosch University who provided technical assistance. A special word of thanks to Elsa van Niekerk, who assisted with the graphics of the paper.

Supplementary material

705_2010_793_MOESM1_ESM.doc (150 kb)
Supplementary material 1 (DOC 150 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • A. E. C. Jooste
    • 1
    Email author
  • H. J. Maree
    • 2
  • D. U. Bellstedt
    • 3
  • D. E. Goszczynski
    • 1
  • G. Pietersen
    • 1
  • J. T. Burger
    • 2
  1. 1.ARC-Plant Protection Research InstitutePretoriaSouth Africa
  2. 2.Department of GeneticsStellenbosch UniversityStellenboschSouth Africa
  3. 3.Department of BiochemistryStellenbosch UniversityStellenboschSouth Africa

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