Molecular detection of porcine kobuviruses in pigs in Korea and their association with diarrhea
- 507 Downloads
Kobuviruses are small, non-enveloped viruses with a single-stranded, positive-sense genomic RNA, belonging to the family Picornaviridae, a highly diverse family of important pathogens of human and other animals. Porcine kobuvirus has been found recently, and consequently, information about the virus is lacking. In this study, we identified porcine kobuviruses from pigs in Korea by RT-PCR, cloning and sequencing, and we showed the existence of genetic diversity among geographically separated porcine kobuviruses through genetic and phylogenetic analysis. Epidemiological studies of porcine kobuvirus linked to diarrhea indicated that porcine kobuvirus infections are endemic in diarrheic pigs in Korea. Statistical analysis of the porcine kobuvirus positive rate between diarrheic and healthy pigs as well as a survey for other enteric pathogens in diarrheic pigs suggests that porcine kobuvirus may play a role as a causative agent of gastroenteritis in pigs.
KeywordsPorcine Epidemic Diarrhea Virus Enteric Pathogen Aichi Virus Porcine Kobuvirus Bacterial Enteric Pathogen
This work was supported by a grant (Code #20070401034009) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.
- 3.International Committee on Taxonomy of Viruses. Virus taxonomy 2009 [cited 2010 April 29]. Available from http://www.ictvonline.org/virusTaxonomy.asp?version = 2009
- 25.Vinjé J, Vennema H, Maunula L, von Bonsdorff CH, Hoehne M, Schreier E, Richards A, Green J, Brown D, Beard SS, Monroe SS, de Bruin E, Svensson L, Koopmans MP (2003) International collaborative study to compare reverse transcriptase PCR assays for detection and genotyping of noroviruses. J Clin Microbiol 41:1423–1433CrossRefPubMedGoogle Scholar