Archives of Virology

, Volume 155, Issue 9, pp 1453–1461 | Cite as

Dengue-2-virus-interacting polypeptides involved in mosquito cell infection

  • Mandar S. Paingankar
  • Mangesh D. Gokhale
  • Dileep N. Deobagkar
Original Article


For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus–host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase β subunit, HSc 70, orisis, prohibitin, tubulin β chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.


West Nile Virus Dengue Hemorrhagic Fever Mosquito Cell Salivary Gland Extract Heat Shock Cognate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge valuable suggestions given by Deepti Deobagkar. We thank A.C. Mishra, National Institute of Virology, Pune, for the facilities and encouragement and Dipankar Chaterjee, Indian Institute of Science, Bangalore, for the MALDI TOF/TOF facility. This research was supported by UGC-CAS and ICMR grants of DND. MSP is a CSIR Senior Research Fellow.

Supplementary material

705_2010_728_MOESM1_ESM.doc (3.9 mb)
Supplementary material (DOC 4008 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Mandar S. Paingankar
    • 1
  • Mangesh D. Gokhale
    • 2
  • Dileep N. Deobagkar
    • 1
    • 3
  1. 1.Molecular Biology Research Laboratory, Department of Zoology, Centre for Advance StudiesUniversity of PunePuneIndia
  2. 2.Department of Medical Entomology and ZoologyNational Institute of VirologyPuneIndia
  3. 3.Goa UniversityGoaIndia

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