Dengue-2-virus-interacting polypeptides involved in mosquito cell infection
For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus–host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase β subunit, HSc 70, orisis, prohibitin, tubulin β chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.
KeywordsWest Nile Virus Dengue Hemorrhagic Fever Mosquito Cell Salivary Gland Extract Heat Shock Cognate
The authors acknowledge valuable suggestions given by Deepti Deobagkar. We thank A.C. Mishra, National Institute of Virology, Pune, for the facilities and encouragement and Dipankar Chaterjee, Indian Institute of Science, Bangalore, for the MALDI TOF/TOF facility. This research was supported by UGC-CAS and ICMR grants of DND. MSP is a CSIR Senior Research Fellow.
- 16.Mendoza YM, Salas-Benito JS, Lanz-Mendoza H, Hernandez-Martinez S, del Angel RM (2002) A putative receptor for dengue virus in mosquito tissues: localization of a 45-kDa glycoprotein. Am J Trop Med Hyg 67:76–84Google Scholar
- 33.Talavera D, Castillo AM, Dominguez MC, Gutierrez AE, Meza I (2004) IL8 release, tight junction and cytoskeleton dynamic reorganization conducive to permeability increase are induced by dengue virus infection of microvascular endothelial monolayers. J Gen Virol 85:1801–1813PubMedCrossRefGoogle Scholar
- 38.Wolfersberger M, Liithy P, Maurer A, Parenti P, Sacchi VF, Giordana B, Hanozel GM (1987) Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae). Comp Biochem Physiol 86:301–308CrossRefGoogle Scholar