Archives of Virology

, Volume 155, Issue 8, pp 1273–1279

Induction of TNF-α in human macrophages by avian and human influenza viruses

  • Yuwarat Monteerarat
  • Saori Sakabe
  • Somying Ngamurulert
  • Sirawat Srichatraphimuk
  • Wasana Jiamtom
  • Kridsada Chaichuen
  • Arunee Thitithanyanont
  • Parichart Permpikul
  • Taweesak Songserm
  • Pilaipan Puthavathana
  • Chairul A. Nidom
  • Le Quynh Mai
  • Kiyoko Iwatsuki-Horimoto
  • Yoshihiro Kawaoka
  • Prasert Auewarakul
Original Article

DOI: 10.1007/s00705-010-0716-y

Cite this article as:
Monteerarat, Y., Sakabe, S., Ngamurulert, S. et al. Arch Virol (2010) 155: 1273. doi:10.1007/s00705-010-0716-y

Abstract

The highly pathogenic avian influenza virus H5N1 is known to induce high level of tumor necrosis factor α (TNF-α) from primary macrophages. However, it is still unclear whether current H5N1 strains also induce high TNF-α production, as most of the data were derived from extinct clade 0 H5N1 strain. Here, we show that current clade 1 and 2 H5N1 strains induce variable levels of TNF-α that are not necessarily higher than those induced by seasonal influenza viruses. The result suggests that hyper-induction of TNF-α in human macrophages is not always associated with a highly pathogenic phenotype. We further tested the contribution of the NS gene segment from H5N1 isolates to TNF-α induction by using reverse genetics. While NS conferred some variation in TNF-α induction when incorporated into an H1N1 virus genetic background, it did not affect TNF-α induction in an H5N1 virus genetic background, suggesting that other viral genes are involved.

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yuwarat Monteerarat
    • 1
  • Saori Sakabe
    • 2
  • Somying Ngamurulert
    • 1
  • Sirawat Srichatraphimuk
    • 1
  • Wasana Jiamtom
    • 1
  • Kridsada Chaichuen
    • 3
  • Arunee Thitithanyanont
    • 4
  • Parichart Permpikul
    • 5
  • Taweesak Songserm
    • 6
  • Pilaipan Puthavathana
    • 1
  • Chairul A. Nidom
    • 7
  • Le Quynh Mai
    • 8
  • Kiyoko Iwatsuki-Horimoto
    • 2
  • Yoshihiro Kawaoka
    • 2
    • 9
    • 10
    • 11
  • Prasert Auewarakul
    • 1
  1. 1.Department of Microbiology, Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand
  2. 2.Division of Virology, Department of Microbiology and Immunology, Institute of Medical ScienceUniversity of TokyoTokyoJapan
  3. 3.Faculty of Veterinary ScienceMahidol UniversityNakhon PathomThailand
  4. 4.Faculty of ScienceMahidol UniversityBangkokThailand
  5. 5.Department of Transfusion Medicine, Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand
  6. 6.Faculty of Veterinary MedicineKasetsart UniversityBangkokThailand
  7. 7.Avian Influenza Laboratory, Tropical Disease CentreAirlannga UniversitySurabayaIndonesia
  8. 8.National Institute of Hygiene and EpidemiologyHanoiVietnam
  9. 9.International Research Center for Infectious Diseases, Institute of Medical ScienceUniversity of TokyoTokyoJapan
  10. 10.Department of Pathobiological SciencesSchool of Veterinary Medicine, University of Wisconsin-MadisonMadisonUSA
  11. 11.ERATO Infection-Induced Host Responses ProjectSaitamaJapan

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