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Archives of Virology

, Volume 155, Issue 7, pp 1047–1057 | Cite as

Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India

  • S. R. Kunkalikar
  • P. Sudarsana
  • P. Rajagopalan
  • Usha B. Zehr
  • K. S. RaviEmail author
Original Article

Abstract

Two isolates of Capsicum chlorosis virus (CaCV, genus Tospovirus) from tomato (CaCV-To-Ind) and chilli (CaCV-Ch-Pan), collected from Haryana and Uttar Pradesh states of northern India respectively, were compared. A comparison of the amino acid sequences of their N genes revealed more than 96% identity, confirming that the virus isolates in India have a high degree of sequence conservation and are closely related to Australian isolates. Analysis of the host range of CaCV revealed no biological difference between the isolates, but they differed from CaCV-Australia. The nucleotide sequences of S, M and L RNA of CaCV-Ch-Pan were determined. The S RNA contains 3,105 nucleotides (nt), with NSs and N genes of 1,320 and 828 nt, respectively. The M RNA consists of 4,821 nt, with an NSm gene of 927 nt and a Gn/Gc gene of 3,366 nt. The intergenic regions of S and M RNA contain 824 and 425 nt, respectively. The L RNA consists of 8,912 nt, with an RNA-dependent RNA polymerase gene of 8,634 nt.

Keywords

Reverse Transcription Polymerase Chain Reaction Australian Isolate Uttar Pradesh Iris Yellow Spot Virus Chlorotic Lesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are thankful to Dr. Shyi Dong Yeh, NCHU, Taiwan, for kindly providing the PAbs against the CaCV N protein and MAbs to the WSMoV-N and NSs proteins. We also thank Dr. Stephen Winter (DSMZ, Braunchewig, Germany) for providing PAbs to the CaCV N protein.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • S. R. Kunkalikar
    • 1
  • P. Sudarsana
    • 1
  • P. Rajagopalan
    • 1
  • Usha B. Zehr
    • 1
  • K. S. Ravi
    • 1
    Email author
  1. 1.Mahyco Research CenterJalnaIndia

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