Archives of Virology

, Volume 155, Issue 7, pp 1047–1057 | Cite as

Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India

  • S. R. Kunkalikar
  • P. Sudarsana
  • P. Rajagopalan
  • Usha B. Zehr
  • K. S. RaviEmail author
Original Article


Two isolates of Capsicum chlorosis virus (CaCV, genus Tospovirus) from tomato (CaCV-To-Ind) and chilli (CaCV-Ch-Pan), collected from Haryana and Uttar Pradesh states of northern India respectively, were compared. A comparison of the amino acid sequences of their N genes revealed more than 96% identity, confirming that the virus isolates in India have a high degree of sequence conservation and are closely related to Australian isolates. Analysis of the host range of CaCV revealed no biological difference between the isolates, but they differed from CaCV-Australia. The nucleotide sequences of S, M and L RNA of CaCV-Ch-Pan were determined. The S RNA contains 3,105 nucleotides (nt), with NSs and N genes of 1,320 and 828 nt, respectively. The M RNA consists of 4,821 nt, with an NSm gene of 927 nt and a Gn/Gc gene of 3,366 nt. The intergenic regions of S and M RNA contain 824 and 425 nt, respectively. The L RNA consists of 8,912 nt, with an RNA-dependent RNA polymerase gene of 8,634 nt.


Reverse Transcription Polymerase Chain Reaction Australian Isolate Uttar Pradesh Iris Yellow Spot Virus Chlorotic Lesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are thankful to Dr. Shyi Dong Yeh, NCHU, Taiwan, for kindly providing the PAbs against the CaCV N protein and MAbs to the WSMoV-N and NSs proteins. We also thank Dr. Stephen Winter (DSMZ, Braunchewig, Germany) for providing PAbs to the CaCV N protein.


  1. 1.
    Bucher E, Sijen T, de Haan P, Goldbach R, Prins M (2003) Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. J Virol 77:1329–1336CrossRefPubMedGoogle Scholar
  2. 2.
    Bag S, Druffel KL, Salewsky T, Pappu HR (2009) Nucleotide sequence and genome organization of the medium RNA of Iris yellow spot virus from the United States. Arch Virol 154:715–718CrossRefPubMedGoogle Scholar
  3. 3.
    Chiemsombat P, Gajanandana O, Warin N, Hongprayoon R, Bhunchoth A, Pongsapich P (2008) Biological and molecular characterization of tospoviruses in Thailand. Arch Virol 153:571–577CrossRefPubMedGoogle Scholar
  4. 4.
    Chu FH, Chao CH, Chung MH, Chen CC, Yeh SD (2001) Completion of the genome sequence of Watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. Phytopathology 91:361–368CrossRefPubMedGoogle Scholar
  5. 5.
    Ciuffo M, Tavella L, Pacifico D, Masenga V, Turina M (2008) A member of a new Tospovirus species isolated in Italy from wild buckwheat (Polygonum convolvulus). Arch Virol 153:2059–2068CrossRefPubMedGoogle Scholar
  6. 6.
    de Haan P, de Avila AC, Kormelink R, Westerbroek A, Gielen JL, Peters D, Goldbach RW (1992) The nucleotide sequence of the S RNA of impatiens necrotic spot virus, a novel tospovirus. FEBS Lett 306:27–32CrossRefPubMedGoogle Scholar
  7. 7.
    de Haan P, Kormelink R, Resende RO, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA-polymerase. J Gen Virol 72:2207–2216CrossRefPubMedGoogle Scholar
  8. 8.
    de Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of Tomato spotted wilt virus has an ambisense character. J Gen Virol 71:1001–1007CrossRefPubMedGoogle Scholar
  9. 9.
    Elliot RM (1990) Molecular biology of the Bynyaviridae. J Gen Virol 71:501–522CrossRefGoogle Scholar
  10. 10.
    Francki RIB, Fauquet CM, Knudson DL, Brown F (1991) Classification and nomenclature of viruses. Arch Virol 2:281–283Google Scholar
  11. 11.
    German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny and vector relationships. Ann Rev Phytopathol 30:315–348CrossRefGoogle Scholar
  12. 12.
    Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R (2006) Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept. Arch Virol 152:85–96CrossRefPubMedGoogle Scholar
  13. 13.
    Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R (2005) A new tomato-infecting tospovirus from Iran. Phytopathology 95:852–858CrossRefPubMedGoogle Scholar
  14. 14.
    Jain RK, Pappu HR, Pappu SS, Krishnareddy M, Vani A (1998) Watermelon bud necrosis tospovirus from India is a distinct virus species belonging to serogroup IV. Arch Virol 43:1637–1644CrossRefGoogle Scholar
  15. 15.
    Jones RAC, Sharman M (2005) Capsicum chlorosis virus infecting Capsicum annuum in East Kimberley region of Western Australia. Aust Plant Pathol 34:397–399CrossRefGoogle Scholar
  16. 16.
    Knierim D, Blawid R, Maiss E (2006) The complete nucleotide sequence of a Capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch Virol 151:1761–1782CrossRefPubMedGoogle Scholar
  17. 17.
    Kormelink R, de Haan P, Meurs C, Peters D, Goldbach R (1992) The nucleotide sequence of the M RNA segment of Tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J Gen Virol 73:2795–2804CrossRefPubMedGoogle Scholar
  18. 18.
    Kormelink R, Storms M, van Lent J, Peters D, Goldbach R (1994) Expression and subcellular location of the NSm protein of Tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200:56–65CrossRefPubMedGoogle Scholar
  19. 19.
    Krishnareddy M, Usha Rani R, AnilKumar KS, MadhaviReddy K, Pappu HR (2008) Capsicum chlorosis virus (Genus Tospovirus) Infecting Chili Pepper (Capsicum annuum) in India. Plant Dis 92:1469CrossRefGoogle Scholar
  20. 20.
    Kunkalikar S, Sudarsana P, Rajagopalan P, Zehr UB, Naidu RA, Ravi KS (2007) First report of Capsicum chlorosis virus in tomato in India. Plant Health Progress. doi: 10.1094/PHP-2007-1204-01-BR
  21. 21.
    McMichael LA, Persley DM, Thomas JE (2002) A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia. Aust Plant Pathol 31:231–239CrossRefGoogle Scholar
  22. 22.
    Moyer JW (1999) Tospoviruses (Bunyaviridae). In: Granoff A, Webster RG (eds) Encyclopedia of virology. Academic Press, New York, pp 1803–1807Google Scholar
  23. 23.
    Okuda M, Taba S, Hanada K (2003) The S RNA segment determines symptom differences on Tetragonia expansa between two Watermelon silver mottle virus isolates. Physiol Mol Plant Pathol 62:327–332CrossRefGoogle Scholar
  24. 24.
    Pappu SS, Bhat AI, Pappu HR, Deom CM, Culbreath AK (2000) Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs. Arch Virol 145:1035–1045CrossRefPubMedGoogle Scholar
  25. 25.
    Persley DM, Thomas JE, Sharman M (2006) Tospovirus-an Australian perspective. Aust Plant Pathol 35:161–180CrossRefGoogle Scholar
  26. 26.
    Polston JE, McGovern RJ, Brown LG (1999) Introduction of Tomato yellow leaf curl virus in Florida and implications for the spread of this and other geminiviruses of tomato. Plant Dis 83:984–988CrossRefGoogle Scholar
  27. 27.
    Premachandra WTSD, Borgemeister C, Maiss E, Knierim D, Poehling HM (2005) Ceratothripoides claratris, a new vector of a Capsicum chlorosis virus isolate infecting tomato in Thailand. Phytopathology 95:659–663CrossRefPubMedGoogle Scholar
  28. 28.
    Qui WP, Geske SM, Hickey CM, Moyer JW (1998) Tomato spotted wilt tospovirus genome reassortment and genome segment-specific adaptation. Virology 244:186–194CrossRefGoogle Scholar
  29. 29.
    Ravi KS, Kitkaru AS, Winter S (2006) Iris yellow spot virus in onions: a new tospovirus record from India. Plant Pathol 55:288CrossRefGoogle Scholar
  30. 30.
    Reddy DVR, Ratna AS, Sudarshana MR, Poul F, Kiran Kumar I (1992) Serological relationships and purification of Peanut bud necrosis virus, a tospovirus occuring in peanut (Arachis hypogaea L.) in India. Ann Appl Biol 120:279–286CrossRefGoogle Scholar
  31. 31.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  32. 32.
    Saritha RK, Jain RK (2007) Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata in India. Arch Virol 152:1195–1200CrossRefPubMedGoogle Scholar
  33. 33.
    Satyanarayana T, Gowda S, Reddy LK, Mitchell SE, Dawson WO, Reddy DVR (1998) Peanut yellow spot virus is a member of a new serogroup of Tospovirus genus based on small (S) RNA sequence and organization. Arch Virol 143:353–364CrossRefPubMedGoogle Scholar
  34. 34.
    Satyanarayana T, Mitchell SE, Reddy DVR, Brown S, Kresovich S, Jarret R, Naidu RA, Demski JW (1996) Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and identity to other tospoviruses. Arch Virol 141:85–98CrossRefPubMedGoogle Scholar
  35. 35.
    Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okuno T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532:75–79CrossRefPubMedGoogle Scholar
  36. 36.
    van Knippenberg I, Goldbach R, Kormelink R (2005) Tomato spotted wilt virus S-segment mRNAs have overlapping 3′ ends containing a predicted stem-loop structure and conserved sequence motif. Virol Res 110:125–131CrossRefGoogle Scholar
  37. 37.
    Varma A (2007) Emergence and re emergence of plant viruses in India: impact and management options. Tenth plant virus epidemeology symposium, Hyderabad, IndiaGoogle Scholar
  38. 38.
    Williams LV, López Lambertini PM, Shohara K, Biderbost EB (2001) Occurrence and geographical distribution of tospovirus species infecting tomato crops. Argent Plant Dis 85:1227–1229CrossRefGoogle Scholar
  39. 39.
    Yeh SD, Chang TF (1995) Nucleotide sequence of the N gene of Watermelon silver mottle virus, a proposed pew member of the genus Tospovirus. Phytopathology 85:58–64CrossRefGoogle Scholar
  40. 40.
    Yeh SD, Sun IJ, Ho HM, Chang TF (1996) Molecular cloning and sequence analysis of the S RNA of Watermelon silver mottle virus. Acta Horti 431:244–259Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • S. R. Kunkalikar
    • 1
  • P. Sudarsana
    • 1
  • P. Rajagopalan
    • 1
  • Usha B. Zehr
    • 1
  • K. S. Ravi
    • 1
    Email author
  1. 1.Mahyco Research CenterJalnaIndia

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