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Archives of Virology

, Volume 155, Issue 4, pp 601–605 | Cite as

Hydroxyurea as an inhibitor of hepatitis C virus RNA replication

  • Akito NozakiEmail author
  • Manabu Morimoto
  • Masaaki Kondo
  • Takashi Oshima
  • Kazushi Numata
  • Shin Fujisawa
  • Takeshi Kaneko
  • Eiji Miyajima
  • Satoshi Morita
  • Kyoko Mori
  • Masanori Ikeda
  • Nobuyuki Kato
  • Katsuaki Tanaka
Brief Report

Abstract

Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.

Keywords

Internal Ribosome Entry Site Human Immunodeficiency Virus Inhibition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank Kazue Yoshihara and Yoshiko Kubushiro for their technical assistance. This work was supported by a grant for the 2008 Strategic Research Project (No. W20012) provided by Yokohama City University, Japan.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Akito Nozaki
    • 1
    • 2
    Email author
  • Manabu Morimoto
    • 1
  • Masaaki Kondo
    • 1
  • Takashi Oshima
    • 1
  • Kazushi Numata
    • 1
  • Shin Fujisawa
    • 3
  • Takeshi Kaneko
    • 4
  • Eiji Miyajima
    • 5
  • Satoshi Morita
    • 6
  • Kyoko Mori
    • 7
  • Masanori Ikeda
    • 7
  • Nobuyuki Kato
    • 7
  • Katsuaki Tanaka
    • 1
  1. 1.Gastroenterological CenterYokohama City University Medical CenterYokohamaJapan
  2. 2.Department of Transfusion MedicineYokohama City University Medical CenterYokohamaJapan
  3. 3.Department of HematologyYokohama City University Medical CenterYokohamaJapan
  4. 4.Respiratory Disease CenterYokohama City University Medical CenterYokohamaJapan
  5. 5.Department of Laboratory Medicine and Clinical InvestigationYokohama City University Medical CenterYokohamaJapan
  6. 6.Department of Biostatistics and EpidemiologyYokohama City University Medical CenterYokohamaJapan
  7. 7.Department of Tumor VirologyOkayama University Graduate School of Medicine, Dentistry, and Pharmaceutical SciencesOkayamaJapan

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