Isolation and characterization of a novel Staphylococcus aureus bacteriophage, ϕMR25, and its therapeutic potential
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A novel bacteriophage, ϕMR25, was isolated from a lysogenic Staphylococcus aureus strain by mitomycin C induction. Its biological features were analyzed in comparison with ϕMR11, which was described previously as a prototype therapeutic phage. ϕMR25 is morphologically similar to ϕMR11 (morphotype B1 of family Myoviridae) but has a broader host range than ϕMR11 on S. aureus strains. ϕMR25 can also multiply on S. aureus lysogens of ϕMR11. Its DNA is 44,342 bp in size, is predicted to include 70 open reading frames, and does not contain genes related to toxin or drug resistance. The lysogenic module and most of the putative virion protein genes are completely different from those of ϕMR11. In spite of their genetic diversity, intraperitoneal administration of ϕMR25 rescued mice inoculated with a lethal dose of S. aureus, as was the case for ϕMR11. These results suggest that ϕMR25 could be another candidate phage to treat S. aureus infection.
KeywordsPhage Genome Major Capsid Protein Aureus Strain Phage Therapy Temperate Phage
This research was partly supported by a grant from the Green Science Project, Kochi University.
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