Archives of Virology

, Volume 155, Issue 4, pp 481–489 | Cite as

Phylogenetic analysis of bovine leukemia viruses isolated in South America reveals diversification in seven distinct genotypes

  • Gonzalo Moratorio
  • Gonzalo Obal
  • Ana Dubra
  • Agustín Correa
  • Sergio Bianchi
  • Alejandro Buschiazzo
  • Juan Cristina
  • Otto Pritsch
Original Article


Bovine leukaemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus of the family Retroviridae. Recent studies revealed that BLV strains can be classified into six different genotypes and raised the possibility that another genotype may exist. In order to gain insight into the degree of genetic variability of BLV strains circulating in the South American region, a phylogenetic analysis was performed using gp51 env gene sequences. The results of these studies revealed the presence of seven BLV genotypes in this geographic region and the suitability of partial gp51 env gene sequences for phylogenetic inference. A significant number of amino acid substitutions found in BLV strains isolated in South America map to the second neutralization domain of gp51. A 3D molecular model of BLV gp51 revealed that these substitutions are located on the surface of the molecule. This may provide a selective advantage to overcome immune host neutralization.


Bovine Leukaemia Virus Bovine Leukaemia Virus Infection South American Region Bovine Leukaemia Virus Strain Neutralization Domain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to thank Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) for support through Project No. FPTA-INIA 174 and the Centre National de la Recherche Scientifique (LIA-LPVL). Authors also acknowledge support by PEDECIBA and Agencia Nacional de Investigación e Innovación (ANII) (Uruguay).

Supplementary material

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Supplementary material 1 (DOC 190 kb)
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Supplementary material 2 (DOC 195 kb)
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Supplementary material 3 (DOC 50 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Gonzalo Moratorio
    • 1
    • 2
  • Gonzalo Obal
    • 1
  • Ana Dubra
    • 1
    • 2
  • Agustín Correa
    • 3
  • Sergio Bianchi
    • 1
    • 4
  • Alejandro Buschiazzo
    • 5
  • Juan Cristina
    • 2
  • Otto Pritsch
    • 1
    • 6
  1. 1.Unidad de Biofísica de ProteínasInstitut Pasteur de MontevideoMontevideoUruguay
  2. 2.Laboratorio de Virología Molecular, Centro de Investigaciones NuclearesFacultad de CienciasMontevideoUruguay
  3. 3.Unidad de Proteínas RecombinantesInstitut Pasteur de MontevideoMontevideoUruguay
  4. 4.Departamento de Fisiopatología, Hospital de ClínicasUniversidad de la RepúblicaMontevideoUruguay
  5. 5.Unidad de Cristalografía de ProteínasInstitut Pasteur de MontevideoMontevideoUruguay
  6. 6.Departamento de Inmunobiología, Facultad de MedicinaUniversidad de la RepúblicaMontevideoUruguay

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